猴B病毒抗体不同检测方法的比对

发布时间：2018-09-11 15:25

【摘要】：目的猴B病毒(monkey B virus,BV),也称猴疱疹病毒I型(Cercopithecine herpesvirus 1),是重要的人兽共患病原。根据国家标准,猴B病毒作为抗原检测抗体,但由于生物安全问题,抗原制备受到很大限制,因此使用替代抗原进行抗体血清学检测并进行比对验证。方法应用2种ELISA方法(抗原分别为BV和HVP2)和1种免疫酶法EIA方法(抗原为HSV-1)对本实验室135份送检恒河猴血液样品进行筛查,对阳性及可疑样品再以免疫荧光法IFA和Western blot方法(抗原为HSV-1)以及以HSV-1 g C1纯化糖蛋白为抗原的免疫印迹方法验证。结果HVP2-ELISA、BV-ELISA和HSV-1-EIA阳性检出率分别为32.6%、37.8%和34.8%;3种方法检测结果一致的样品占91.1%(123/135),阳性结果可被IFA和WB确证;可疑样品12份,33.3%(4/12)的样品经验证检验为阳性。结论与BV抗原相比,替代抗原HSV-1的敏感性和特异性较HVP2更为接近;阳性样品及可疑样品的确证检验应使用多种方法,避免漏检。
[Abstract]:Objective simian B virus (monkey B virus,BV), also known as simian herpesvirus type I (Cercopithecine herpesvirus 1), is an important zoonotic pathogen. According to the national standard, simian B virus is used as antigen to detect antibody, but because of biosafety problem, antigen preparation is restricted greatly. Methods two ELISA methods (antigens BV and HVP2) and one immunoenzymatic EIA method (antigen HSV-1) were used to screen 135 rhesus monkey blood samples. The positive and suspicious samples were confirmed by immunofluorescence IFA and Western blot (antigen as HSV-1) and HSV-1 g C1 purified glycoprotein as antigen by Western blotting. Results the positive rates of HVP2-ELISA,BV-ELISA and HSV-1-EIA were 37.8% (37.8%) and 34.828% (34.8%), respectively. 91.1% (123 / 135) of the samples had the same results. The positive results could be confirmed by IFA and WB, and 12 samples of suspicious samples (33.3%) were positive. Conclusion compared with BV antigen, the sensitivity and specificity of substitute antigen HSV-1 is closer than that of HVP2.
【作者单位】：中国医学科学院医学实验动物研究所北京协和医学院比较医学中心;
【基金】：协和青年基金(编号:3332015196) 卫计委公益性行业科研专项(编号:201302006)
【分类号】：R392

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