大肠杆菌可溶性表达人鳞状上皮细胞癌抗原的制备及应用

发布时间：2018-09-13 16:08

【摘要】：旨在建立基于大肠杆菌表达系统的高效可溶性表达人鳞状上皮细胞癌抗原(SCCAg)方法,获得具有较好活性的重组SCCAg抗原并应用于建立抗原检测方法。基于pGEX-6P-1载体和大肠杆菌E.coliER2566菌株开展重组SCCAg抗原可溶性表达纯化方法研究,评价纯化抗原活性,筛选特异性单克隆抗体,初步建立并评价SCCAg抗原检测方法。结果显示,pGEX-6P-1载体和E.coliER2566菌株可用于建立较高效的可溶性表达和纯化SCCAg抗原的方法,获得了具有较高纯度和活性的重组SCCAg抗原,筛选获得特异性单克隆抗体并初步建立了SCCAg管式化学发光检测方法。建立了有效的基于大肠杆菌表达系统的可溶性表达和纯化SCCAg的方法。
[Abstract]:The aim of this study was to establish an efficient and soluble (SCCAg) method for expression of human squamous cell carcinoma antigen (SCCAg) based on Escherichia coli expression system. The recombinant SCCAg antigen with good activity was obtained and applied to the detection of human squamous cell carcinoma antigen. The methods of soluble expression and purification of recombinant SCCAg antigen were studied based on pGEX-6P-1 vector and Escherichia coli E.coliER2566 strain, the purified antigen activity was evaluated, the specific monoclonal antibody was screened, and the detection method of SCCAg antigen was preliminarily established and evaluated. The results showed that pGEX-6P-1 vector and E.coliER2566 strain could be used to establish a more efficient method for the expression and purification of SCCAg antigen. The recombinant SCCAg antigen with high purity and activity was obtained. The specific monoclonal antibody was screened and the SCCAg tube chemiluminescence assay was established. An effective method of soluble expression and purification of SCCAg based on Escherichia coli expression system was established.
【作者单位】：厦门大学公共卫生学院分子疫苗学与分子诊断学国家重点实验室国家传染病诊断试剂与疫苗工程技术研究中心;厦门万泰凯瑞生物技术有限公司;
【基金】：国家高技术研究发展计划(“863”计划)(2011AA02A101)
【分类号】：R392

【相似文献】