不同感染途径致大鼠肺炎模型制备的比较

发布时间：2018-09-14 07:07

【摘要】：目的探索铜绿假单胞菌致大鼠肺炎模型的制备方法,为后续研究奠定实验基础。方法将Sprague-Dawley(SD)大鼠48只随机分为4组:A对照组、B气管注射组、C气管插管感染组和D滴鼻感染组。适应饲喂3 d后,分别给予不同造模干预,分别于干预后5、10和15 d动态监测各亚组大鼠体重、体温、白细胞数、肺组织病理学变化等。结果各模型组大鼠行为学表现、体重、体温、白细胞、肺组织炎症病理学表现均与对照组差异有显著性;(2)各模型组均证实为铜绿假单胞菌感染,对照组则阴性。结论经滴鼻途径以铜绿假单胞菌感染大鼠,可得到合格大鼠肺炎模型;此感染途径可避免手术创口带来的炎症反应干扰,简便易行,可推广。
[Abstract]:Objective to investigate the preparation of rat pneumonia model induced by Pseudomonas aeruginosa. Methods 48 Sprague-Dawley (SD) rats were randomly divided into 4 groups: control group (n = 4), tracheal intubation group (n = 4) and group D nasal drip (n = 4). The rats were fed with different models for 3 days. The body weight, body temperature, white blood cell count, lung histopathological changes were dynamically monitored on the 10th and 15th days after the intervention. Results there were significant differences in behavior, body weight, body temperature, white blood cell, lung inflammation and pathology between each model group and control group. (2) Pseudomonas aeruginosa infection was found in all model groups, but negative in control group. Conclusion A qualified rat model of pneumonia can be obtained by infecting rats with Pseudomonas aeruginosa via nasal drip, which can avoid the interference of inflammatory reaction caused by surgical wound, be simple and can be popularized.
【作者单位】：首都医科大学附属北京中医医院北京市中医研究所中医感染性疾病基础研究北京市重点实验室;首都医科大学附属北京中医医院;
【基金】：国家自然科学基金项目(No.81503399,No.81373813) 国家“重大新药创制”科技重大专项(No.2013ZX09102026) 首都医科大学附属北京中医医院院级课题(No.YJ-201734) 首都医科大学基础-临床科研合作基金(No.16JL74)
【分类号】：R-332;R563.1

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