外源性EGF对中年大鼠运动活性及纹状体多巴胺含量的影响

发布时间：2018-09-17 15:08

【摘要】：目的:探讨表皮生长因子(EGF)对中年大鼠运动活性以及纹状体多巴胺含量的影响。方法:经皮给予健康18月龄大鼠不同剂量EGF(0.01,0.05,0.1,0.15,0.3 mg/ml),通过旷场实验检测大鼠运动行为的改变,通过液相色谱-串联质谱分析多巴胺的含量变化。结果:与对照组相比,小剂量EGF组大鼠(0.01,0.05 mg/ml)旷场实验中各行为参数没有变化;中等剂量EGF组大鼠(0.1,0.15 mg/ml)旷场实验中闻嗅行为、探索行为、趋触性行为、运动行为和理毛行为改善;大剂量EGF(0.3 mg/m1)组大鼠旷场实验中各行为参数下降。纹状体多巴胺及其代谢产物DOPAC和HVA的含量与对照组相比,小剂量EGF(0.01 mg/rnl)组开始上升,中等剂量EGF(0.1 mg/ml)组达到峰值,大剂量EGF(0.3 mg/ml)组下降。结论:EGF对中年大鼠运动活性以及纹状体多巴胺的含量均有影响。
[Abstract]:Aim: to investigate the effects of epidermal growth factor (EGF) on motor activity and dopamine content in striatum of middle-aged rats. Methods: healthy 18-month-old rats were given different doses of EGF (0.010.05U 0.150.30 mg/ml). The changes of exercise behavior and dopamine content were detected by open-field experiment and HPLC / tandem mass spectrometry respectively. The changes of dopamine content in rats were analyzed by HPLC and tandem mass spectrometry (LC-MS). Results: compared with the control group, there was no change in the behavioral parameters in the open field test of the rats in the low dose EGF group (0. 01 卤0. 05 mg/ml), while in the middle dose EGF group (0. 1 0. 15 mg/ml), the sniffing behavior, exploratory behavior, contact behavior, motor behavior and hairdressing behavior of the rats in the middle dose EGF group (0. 1 0. 15 mg/ml) were improved. The behavioral parameters of large dose EGF (0. 3 mg/m1) group were decreased in open field experiment. Compared with the control group, the contents of dopamine and its metabolites DOPAC and HVA in striatum began to increase in the low dose EGF (0. 01 mg/rnl) group, reached the peak in the middle dose EGF (0. 1 mg/ml) group, and decreased in the high dose EGF (0. 3 mg/ml) group. Conclusion: EGF has an effect on motor activity and dopamine content in striatum of middle-aged rats.
【作者单位】：河北医科大学解剖教研室;南阳市第二人民医院影像科;
【基金】：河北省卫生厅青年科技基金(20090310)
【分类号】：R339.38

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