黄芪桂枝五物汤对CIA模型大鼠关节滑膜细胞凋亡的影响

发布时间：2018-10-04 23:10

【摘要】：目的:研究黄芪桂枝五物汤(HGW)对胶原诱导型关节炎(CIA)模型大鼠关节滑膜细胞凋亡的影响,并探讨其作用机制。方法:选用SD大鼠72只,随机分为正常组,模型组,HGW低、中、高剂量组,Tofacitinib(TFN)组,HGW低、中、高剂量组药物剂量分别为16,32,64 g·kg~(-1),相当于临床常人用量的3,6,12倍,TFN给药剂量为15 mg·kg~(-1),正常组、模型组每日给予10 m L·kg~(-1)蒸馏水。以牛Ⅱ型胶原加弗氏完全佐剂建立CIA大鼠模型,经HGW治疗4周后光镜下观察各组大鼠踝关节病理形态;通过流式细胞术检测白细胞介素(IL)-4,IL~(-1)0含量,免疫组化法检测B淋巴细胞瘤-2(B-cell lymphoma-2,Bcl-2),Bcl-2相关X蛋白(Bax)蛋白表达,DNA断裂的原位末端标记法(TUNEL)染色检测滑膜细胞的凋亡。结果:与正常组比较,模型组,HGW低、中、高剂量组,TFN组大鼠滑膜中Bcl-2蛋白表达增多(P0.05),模型组Bax蛋白表达减少(P0.05);与模型组比较,HGW高剂量组,TFN组大鼠滑膜中Bcl-2蛋白表达减少,Bax蛋白表达增多(P0.05)。滑膜细胞凋亡可见,与正常组比较,模型组,HGW低、中、高剂量组,TFN组滑膜细胞增多,凋亡率下降(P0.05);与模型组比较,HGW低、中、高剂量组,TFN组大鼠滑膜细胞凋亡率上升(P0.05);与HGW低剂量组比较,HGW高剂量组滑膜细胞凋亡率显著上升(P0.01);与HGW-M组比较,HGW高剂量组滑膜细胞凋亡率上升(P0.05)。结论:黄芪桂枝五物汤可以促进RA大鼠异常亢进的滑膜细胞凋亡。
[Abstract]:Aim: to study the effect of Huangqi Guizhi Wuwu decoction (HGW) on apoptosis of synovial cells in (CIA) rats with collagen-induced arthritis and its mechanism. Methods: Seventy-two SD rats were randomly divided into normal group, model group with low HGW, medium dose group with low HGW, high dose group with low dose of Tofacitinib (TFN), and high dose group with drug dose of 1632g kg~ (-1). The model group was given 10 mL kg~ (-1) distilled water daily. The CIA rat model was established with bovine collagen 鈪，

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