肝纤溶颗粒与复方鳖甲软肝片对大鼠肝纤维化模型细胞凋亡及内质网应激相关因子表达影响研究

发布时间：2018-10-10 16:18

【摘要】：目的探究肝纤溶颗粒与复方鳖甲软肝片对大鼠肝纤维化模型细胞凋亡及内质网应激相关因子表达影响。方法 48只雄性清洁级SD大鼠随机分为:正常对照组、模型对照组、复方鳖甲软肝片组及肝纤溶颗粒组,每组各12只。除正常对照组外各组复制肝纤维化模型。于复制模型后的3周各组大鼠同时给药,正常对照组与模型对照组大鼠给予生理盐水1.5 ml/kg灌胃,1次/d;复方鳖甲软肝片组给予33.3%复方鳖甲软肝片溶液1.5 ml/kg重灌胃,1次/d;肝纤溶颗粒组给予33.3%肝纤溶颗粒溶液1.5 ml/kg灌胃,1次/d。各组连续用药6周。实验开始后第3、6和9周对各组大鼠肝脏病理学、细胞凋亡数量、肝纤维化指标水平以及内质网应激相关指标进行检测。结果与其他各组相比,肝纤溶颗粒组纤维增生程度评分下降(P0.05),肝凋亡数下降(P0.05);透明质酸、层黏连蛋白、血清Ⅲ型前胶原、血清Ⅳ型胶原水平下降(P0.05);CHOP、ATF6蛋白表达水平下降(P0.05)。结论肝纤溶颗粒组肝纤维化大鼠细胞凋亡数量和肝纤维化水平较复方鳖甲软肝片组下降,差异有统计学意义(P0.05),肝纤溶颗粒能够改善肝脏病理变化,调节内质网应激相关指标,对临床有指导意义。
[Abstract]:Objective to investigate the effects of Ganxian granule and compound Biejia Ruangan tablet on apoptosis and expression of ER stress related factors in rat liver fibrosis model. Methods Forty-eight male SD rats of clean grade were randomly divided into normal control group, model control group, compound Biejia Ruanggan tablet group and Ganxian granule group with 12 rats in each group. Hepatic fibrosis model was established in each group except the normal control group. Rats in each group were given drugs at the same time at 3 weeks after the establishment of the model. Normal control group and model control group were given normal saline 1.5 ml/kg intragastric perfusion once a day, compound Biejia Ruanggan tablet group was given 33.3% compound Biejia Ruanggan tablet solution 1.5 ml/kg reperfused once a day, and liver fibrinolytic granule group was given 33.3% liver fibrinolytic granule solution 1.5 ml/kg intragastrically, once a day. Each group was treated continuously for 6 weeks. Liver pathology, apoptosis, liver fibrosis and endoplasmic reticulum stress were measured at the 6th and 9th week after the experiment. Results compared with other groups, hepatic fibrinolytic granule group had lower grade of fibroproliferative degree (P0.05) and lower number of apoptosis (P0.05), hyaluronic acid, laminin, serum type 鈪，

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