鲍曼不动杆菌Diversilab（？）系统分型方法的建立与评价

发布时间：2018-10-12 14:54

【摘要】：目的：应用DiversiLab系统对鲍曼不动杆菌进行基因分型,并系统评价DiversiLab系统、多位点序列分析技术、脉冲场凝胶电泳技术的分辨率和三种方法的一致性分析。方法：对2005年全国15个城市医院临床分离的89株鲍曼不动杆菌,应用以rep-PCR为原理的DiversiLab系统分型技术进行分型,并与脉冲场凝胶电泳分型和多位点序列分析技术结果进行比较；应用Ridom EpiCompare Version 1.0软件得出Simpson相关系数及可信区间、AR系数和Wallace系数等,从而更精确地比较三种方法的分辨力和一致性分析。结果： DiversiLab系统将89株鲍曼不动杆菌分为8簇流行克隆和28个散发克隆；多位点序列分型将其分为39个序列型,并归为一个克隆复合体CC92 (Clone Complex 92)和47个单一体(Singleton);脉冲场凝胶电泳将其分成5个流行脉冲型和37个散发克隆。DiversiLab系统、MLST和PFGE的Simpson相关系数分别为0.899,0.894和0.934。AR (MLST-PFGE)=0.502, AR (DiversiLab-PFGE) =0.479, AR (DiversiLab-MLST)=0.434,从AR值来看,MLST和PFGE的相关性最好。对于DiversiLab系统而言,与PFGE分型结果的一致性比其与MLST分型结果的一致性好。Wallace系数中,W1 (PFGE-MLST)=0.709, W1 (PFGE-DiversiLab)=0.659。因此,可由PFGE结果大致推测出MLST和DiversiLab系统分型结果。结论：在鲍曼不动杆菌的分子分型方法中,DiversiLab系统具备较高的分辨力和准确性,与PFGE分型结果的一致性比与MLST的一致性好；且其操作快速、简单,可重复性好,可用于快速或大量菌株的首选分型方法。
[Abstract]:Objective: to study the genotyping of Acinetobacter baumannii by DiversiLab system, and to evaluate the resolution of DiversiLab system, multi-locus sequence analysis, pulse field gel electrophoresis and consistency analysis of three methods. Methods: 89 strains of Acinetobacter baumannii isolated from 15 hospitals in 2005 were classified by DiversiLab system based on rep-PCR. The results were compared with the results of pulse field gel electrophoresis typing and multilocus sequence analysis, and the Simpson correlation coefficient and confidence interval, AR coefficient and Wallace coefficient were obtained by Ridom EpiCompare Version 1.0 software. Thus, the resolution and consistency analysis of the three methods are compared more accurately. Results: 89 strains of Acinetobacter baumannii were divided into 8 clusters of epidemic clones and 28 sporadic clones by DiversiLab system. A clone complex CC92 (Clone Complex 92) and 47 monosomes (Singleton); pulse field gel electrophoresis were divided into 5 popular pulse types and 37 sporadic clones. The Simpson correlation coefficients of MLST and PFGE were 0.899N0.894 and 0.502, AR (DiversiLab-PFGE) = 0.479, AR (DiversiLab-MLST) = 0.434, respectively. In terms of AR, MLST and PFGE have the best correlation. For DiversiLab system, the consistency with PFGE typing results is better than that with MLST typing results. In Wallace coefficient, W1 (PFGE-MLST) = 0.709, W1 (PFGE-DiversiLab) = 0.659. Therefore, the results of MLST and DiversiLab system typing can be inferred from PFGE results. Conclusion: among the molecular typing methods of Acinetobacter baumannii, the DiversiLab system has higher resolution and accuracy, better consistency with the results of PFGE typing than with MLST, and its operation is fast, simple and reproducible. It can be used as the preferred typing method for rapid or large number of strains.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R378

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