鸟苷酸结合蛋白2调控β-葡聚糖诱导的小鼠树突状细胞成熟
发布时间：2018-10-16 12:32
【摘要】：目的研究鸟苷酸结合蛋白2(GBP2)对树突状细胞(DC)成熟和功能的影响。方法通过基因芯片和实时定量PCR研究β-葡聚糖诱导的DC基因的变化情况,转染GBP2小干扰RNA(si RNA),敲低GBP2的表达,流式细胞术检测DC表面CD11c、主要组织相容性复合体Ⅱ(MHC-Ⅱ)、CD80水平,ELISA检测细胞上清中白细胞介素6(IL-6)、IL-12p70、TNF-α和IL-10水平。T细胞增殖实验检测全葡聚糖颗粒(WGP)刺激的成熟DC在转染GBP2-si RNA后,对卵清蛋白(OVA)特异性CD4+T细胞的促增殖效应。结果β-葡聚糖诱导后,DC的GBP2 m RNA水平显著降低;敲低DC的GBP2水平后,其表面CD11c、主要组织相容性复合体Ⅱ(MHC-Ⅱ)、CD80水平降低,同时,IL-6、IL-12、TNF-α分泌下降;经WGP刺激的成熟DC在转染GBP2-si RNA后,对OVA特异性CD4+T细胞的促增殖效应降低。结论 GBP2能调控β-葡聚糖诱导的DC成熟,进一步抑制T细胞的增殖。
[Abstract]:Objective to study the effects of guanylate binding protein 2 (GBP2) on (DC) maturation and function in dendritic cells. Methods the changes of DC gene induced by 尾 -glucan were studied by gene chip and real-time quantitative PCR, and the expression of GBP2 was reduced by GBP2 small interference RNA (si RNA), knockout. The levels of CD11c, major histocompatibility complex 鈪，
本文编号：2274373
[Abstract]:Objective to study the effects of guanylate binding protein 2 (GBP2) on (DC) maturation and function in dendritic cells. Methods the changes of DC gene induced by 尾 -glucan were studied by gene chip and real-time quantitative PCR, and the expression of GBP2 was reduced by GBP2 small interference RNA (si RNA), knockout. The levels of CD11c, major histocompatibility complex 鈪，
本文编号：2274373
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