二至丸对更年期综合征模型大鼠的实验研究

发布时间：2018-10-17 10:39

【摘要】：目的:研究二至丸对更年期模型大鼠的影响。方法:将SPF级雌性SD大鼠60只随机分成假手术对照组、模型对照组、更年欣胶囊组(4mg/kg)、二至丸高(600mg/kg)、中(300mg/kg)、低(150mg/kg)剂量组,各组灌胃给予相应的药物连续30d。除假手术对照组外,给药30d后对其余5组大鼠通过手术将0.2ml无水乙醇注射到卵巢内1次,建立更年期综合征模型大鼠。观察大鼠给药前后体质量的变化,测定血清中雌二醇(E2)、促卵泡成熟激素(FSH)、黄体生成素(LH)、催乳素(PRL)水平,计算子宫系数和阴道上皮细胞角化率,以及测定下丘脑5-羟色胺(5-HT)、去甲肾上腺素(NE)、多巴胺(DA)、5-羟吲哚乙酸(5-HIAA)的含量。结果:与假手术对照组比较,模型对照组大鼠的体质量缓慢降低,肛温升高(P0.05),FSH、LH和PRL水平明显升高(P0.05),E2水平明显减少(P0.01);阴道上皮细胞角化率和子宫系数明显下降(P0.01);降低下丘脑5-HIAA/5-HT(P0.05),增加NE/DA(P0.05);与模型对照组相比较,二至丸各剂量组大鼠体质量增长较模型组快,肛温下降(P0.05);FSH、LH水平明显降低(P0.01);PRL水平明显降低(P0.05),E2水平明显升高(P0.05);阴道上皮细胞角化率明显提高(P0.01);子宫系数增加(P0.05);增加下丘脑5-HIAA/5-HT(P0.05),减少NE/DA(P0.05)。结论:二至丸能既可以改善性激素,又可以增加更年期综合征模型大鼠下丘脑单胺类神经递质含量,从而达到缓解更年期症状的作用。
[Abstract]:Objective: to study the effect of Erzhi pill on climacteric model rats. Methods: sixty female SD rats of SPF grade were randomly divided into sham operation control group, model control group, Gennianxin capsule group (4mg/kg), Erzhi pills high (600mg/kg), middle (300mg/kg) and low (150mg/kg) dosage groups. In addition to the sham operation control group, the other five groups were injected 0.2ml anhydrous alcohol into the ovary once a time after 30 days of administration to establish the climacteric syndrome model rats. The changes of body mass before and after administration were observed. Serum estradiol (E2), follicle maturation hormone (FSH),) luteinizing hormone (LH),) prolactin (PRL), uterine coefficient and vaginal epithelial keratinization rate were measured. The contents of 5-hydroxytryptamine (5-HT) and norepinephrine (NE),) dopamine (DA), 5-hydroxyindoleacetic acid (5-HIAA) in hypothalamus were determined. Results: compared with the sham operation control group, the body mass of the model control group decreased slowly. Anus temperature increased (P0.05), FSH,LH and PRL levels increased significantly (P0.05), E2 level significantly decreased (P0.01), vaginal epithelial cell keratinization rate and uterine coefficient significantly decreased (P0.01), hypothalamic 5-HIAA/5-HT (P0.05), increased NE/DA (P0.05); compared with the model control group, The weight of rats in Erzhi pills group increased faster than the model group, the anus temperature decreased (P0.05); the level of FSH,LH decreased significantly (P0.05); the level of E2 increased significantly (P0.05); the rate of keratinization of vaginal epithelial cells increased significantly (P0.01); the uterine coefficient increased (P0.05); the hypothalamus 5-HIAA/5-HT (P0.05) decreased NE/DA (P0.05). Conclusion: Erzhi pills can not only improve sex hormones but also increase the content of monoamine neurotransmitters in hypothalamus of rats with climacteric syndrome.
【作者单位】：南阳医学高等专科学校中医系;南阳医学高等专科学校护理系;广州中医药大学;
【分类号】：R285.5;R-332

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