复方苦参注射液对乳腺癌模型大鼠的改善作用

发布时间：2018-10-20 08:55

【摘要】：目的研究复方苦参注射液对乳腺癌模型大鼠的改善作用。方法按照随机数字法选雄性SD大鼠15只为正常组,余采用腹腔注射甲基亚硝基脲(MNU)复制大鼠乳腺癌模型。按随机数字表法将造模成功大鼠随机分为5组:模型组,对照组,大中小3个剂量实验组,各15只。从造模成功第2天开始,实验组腹腔注射复方苦参注射液80,40,20 m L·kg~(-1),模型组灌胃三苯氧胺混悬液40 m L·kg~(-1),对照组和正常组大鼠灌胃生理盐水4 m L·kg~(-1),每组均1次/天。干预9周后,用酶联免疫吸附法测定大鼠血清中的雌二醇(E2)与存活素含量、乳腺组织内的细胞增殖抗原标记物(Ki67)、抑癌基因p53(P53)及组织和血清的程序性细胞死亡因子4(PDCD4)水平。结果正常组、对照组、小中大3个剂量实验组和模型组的E2含量分别为(36.92±1.28),(70.09±1.91),(64.40±2.77),(40.49±2.17),(40.49±2.39),(49.56±1.27)ng·L~(-1);这6组的Ki67分别为(15.72±0.76),(70.25±1.59),(65.41±1.33),(21.03±1.50),(21.30±1.10),(40.91±1.85)ng·L~(-1);这6组的P53分别为(10.63±0.89),(50.41±1.73),(45.04±2.09),(11.83±1.29),(12.18±1.48),(28.37±1.66)ng·L~(-1);这6组的存活素分别为(5.95±0.46),(63.91±1.96),(30.85±1.63),(16.96±0.53),(16.58±0.95),(25.36±1.51)ng·L~(-1);这6组的组织PDCD4分别为(101.06±2.49),(31.12±1.37),(35.25±1.13),(79.62±1.73),(79.52±1.45),(56.40±2.82)ng·L~(-1);这6组的血清PDCD4分别为(22.00±1.56),(6.93±0.62),(7.78±0.67),(19.92±1.01),(19.13±4.42),(15.34±1.02)ng·L~(-1)。与对照组相比,中高2个剂量实验组与模型组血清E2、存活素、组织Ki67与P53水平降低,差异有统计学意义(均P0.05);与模型组比较,中高2个剂量实验组的血清E2、存活素、组织Ki67与P53水平较低,差异有统计学意义(均P0.05)。与对照组比较,中高2个剂量实验组与模型组组织与血清PDCD4水平较高,差异有统计学意义(P0.05);与模型组比较,中高2个剂量实验组的组织与血清PDCD4水平较高,差异有统计学意义(P0.05)。结论复方苦参注射液能够明显改善乳腺癌模型大鼠Ki67、存活素、P53及PDCD4水平。
[Abstract]:Objective to study the effect of compound Sophora flavescens injection on breast cancer model rats. Methods Fifteen male SD rats were selected as normal group according to random number method. The breast cancer model was induced by intraperitoneal injection of (MNU). The successful rats were randomly divided into 5 groups: model group, control group, three dose experimental groups, each with 15 rats. From the second day after the successful establishment of the model, the experimental group was intraperitoneally injected with compound Sophora flavescens injection 80 (40g / ml) kg~ (-1), the model group was administrated with 40 mL kg~ (-1) of tamoxifen suspension, and the rats in the control group and the normal group were given 4 mL kg~ (-1) intragastrically, once a day in each group. After 9 weeks of intervention, the levels of estradiol (E2) and survivin in serum, cell proliferation antigen marker (Ki67) in mammary gland, p53 gene p53 and programmed cell death factor 4 (PDCD4) in tissue and serum were measured by enzyme-linked immunosorbent assay (Elisa). Results normal group, control group, 灏忎腑澶，

本文编号：2282662