参茸护肤乳对亚急性皮肤衰老模型小鼠皮肤的影响

发布时间：2018-10-22 12:40

【摘要】：目的观察参茸护肤乳对皮肤衰老的保护作用及可能作用机制。方法 71只小鼠按抽签法分为空白组、模型组、基质组、阳性对照组及参茸高、中、低剂量组各9只。除空白组外,其余各组通过皮下注射D-半乳糖建立皮肤衰老模型。造模后基质组涂抹护肤乳液基质;阳性对照组涂抹复颜滋润抗皱乳液;参茸高、中、低剂量组分别涂抹含12%人参提取物和11%鹿茸胶原多肽的护肤乳、含7%人参提取物和5%鹿茸胶原多肽的护肤乳、含3%人参提取物和2%鹿茸胶原多肽的护肤乳;各组给药剂量均为1 mg/cm~2,共给药42天。观察各组小鼠皮肤组织中的水分、透明质酸、羟脯氨酸、丙二醛(MDA)含量及谷胱甘肽过氧化氢酶(GSH-Px)、超氧化物歧化酶(SOD)活性,另外取8只小鼠进行急性多次皮肤刺激性实验观察参茸护肤乳对小鼠皮肤的刺激性。结果参茸护肤乳对皮肤安全、无刺激性。模型组小鼠皮肤中水分、透明质酸和羟脯氨酸含量及GSH-Px、SOD活力均下降,MDA含量升高(P0.01)。小鼠皮肤经涂抹高剂量参茸护肤乳后,小鼠皮肤的水分、透明质酸和羟脯氨酸的含量及SOD、GSH-Px活性显著升高,MDA含量显著降低(P0.05或P0.01),且与复颜滋润抗皱乳液效果相当。结论参茸护肤乳具有明显改善皮肤衰老的作用,提高皮肤衰老小鼠的皮肤水分、透明质酸、羟脯氨酸含量及SOD、GSH-Px活性,降低MDA含量可能是其机制之一。
[Abstract]:Objective to observe the protective effect of ginseng antler skin care cream on skin aging and its possible mechanism. Methods 71 mice were divided into blank group, model group, matrix group, positive control group and high, middle and low dosage groups according to drawing lots. Skin aging model was established by subcutaneous injection of D-galactose except blank group. After modeling, the matrix group was smeared with skin care emulsion; the positive control group was smeared with complexion moisturizing and anti-wrinkle emulsion; the high, medium and low dosage groups of ginseng were smeared with 12% ginseng extract and 11% velvet antler collagen polypeptide, respectively. The skin care lotion containing 7% ginseng extract and 5% velvet antler collagen polypeptide, 3% ginseng extract and 2% velvet collagen polypeptide skin care cream were given 1 mg/cm~2, for 42 days. The contents of water, hyaluronic acid, hydroxyproline, malondialdehyde (MDA), glutathione catalase (GSH-Px) and superoxide dismutase (SOD) in skin tissue of each group were observed. In addition, 8 mice were used to observe the skin irritation of ginseng antler skin care cream. Results the ginseng antler skin care cream is safe to the skin and has no irritation. In the model group, the content of water, hyaluronic acid and hydroxyproline and the activity of GSH-Px,SOD decreased, but the content of MDA increased (P0.01). The content of water, hyaluronic acid and hydroxyproline and the activity of SOD,GSH-Px were significantly increased, and the content of MDA was significantly decreased (P0.05 or P0.01), and the effect of moisturizing and anti-wrinkle emulsion was similar to that of complexion moisturizing and anti-wrinkle emulsion. Conclusion ginseng antler skin care cream can obviously improve skin senescence, increase skin moisture, hyaluronic acid, hydroxyproline content and SOD,GSH-Px activity of skin aging mice, and decrease MDA content may be one of its mechanisms.
【作者单位】：吉林农业大学中药材学院;长春科技学院医药学院;
【基金】：吉林省科技发展计划(20140204063YY,20160204004YY)
【分类号】：R285.5;R-332

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