CIK对SGC-7901胃癌移植瘤小鼠模型体内抗肿瘤作用的实验研究

发布时间：2018-10-22 17:19

【摘要】：目的探讨细胞因子诱导杀伤细胞(CIK)对胃癌移植瘤小鼠模型的抗肿瘤作用并探讨其作用机制。方法体内实验选取60只C57BL/6小鼠分为模型对照组、5-Fu组、CIK大剂量、中剂量、小剂量组和正常对照组(n=10),分别治疗周期为4周,比较各组胃癌小鼠治疗期间移植瘤生长情况,测定各组小鼠血清和肿瘤组织MMP-9、VEGF及bFGF水平。结果 1)CIK中剂量组小鼠瘤体积于治疗后1周、2周、3周和4周时均较CIK小剂量组显著减少(P0.05),CIK大剂量组小鼠瘤体积治疗后1周、2周、3周和4周时均较CIK中剂量组显著减少(P0.05);2)治疗4周时CIK中剂量组小鼠血清MMP-9、VEGF和bFGF水平均较小剂量组小鼠显著降低(P0.05),CIK大剂量组小鼠血清MMP-9、VEGF和bFGF水平均较中剂量组小鼠显著降低(P0.05);3)治疗4周时CIK中剂量组小鼠移植瘤组织MMP-9、VEGF和bFGF mRNA和蛋白水平均较小剂量组小鼠显著降低(P0.05),CIK大剂量组小鼠移植瘤组织MMP-9、VEGF和bFGF mRNA和蛋白水平均较中剂量组小鼠显著降低(P0.05)。结论细胞因子诱导杀伤细胞可显著抑制SGC-7901胃癌小鼠模型瘤组织的生长,并且降低血液及癌组织中MMP-9、VEGF和bFGF的表达。
[Abstract]:Objective to investigate the antitumor effect of cytokine induced killer cell (CIK) on gastric cancer transplanted tumor mice and its mechanism. Methods Sixty C57BL/6 mice were divided into three groups: model control group, 5-Fu group, high dose, middle dose of CIK, small dose group and normal control group (n = 10). The treatment period was 4 weeks, respectively. The growth of transplanted tumor was compared during the treatment of gastric cancer mice in each group. The levels of MMP-9,VEGF and bFGF in serum and tumor tissue of each group were measured. Results 1) the tumor volume in CIK medium dose group was significantly lower than that in CIK small dose group at 1 week, 2 weeks, 3 weeks and 4 weeks after treatment (P0.05). The tumor volume in), CIK group was significantly lower than that in CIK medium dose group at 1 week, 2 weeks, 3 weeks and 4 weeks after treatment. The levels of serum MMP-9,VEGF and bFGF in the CIK medium dose group were significantly lower than those in the low dose group at 4 weeks after treatment (P0.05) (P0.05) the serum MMP-9,VEGF and bFGF levels in the high dose group were significantly lower than those in the middle dose group (P0.05); 3) at 4 weeks after treatment, the level of CIK in the medium dose group was significantly lower than that in the middle dose group (P0.05). The levels of MMP-9,VEGF and bFGF mRNA and protein in transplanted tumor tissue of mice in the group B were significantly lower than those in the group B (P0.05). The levels of MMP-9,VEGF and bFGF mRNA and protein in the transplanted tumor tissue of the high-dose), CIK group were significantly lower than those of the mice in the middle dose group (P0.05). Conclusion Killer cells induced by cytokines can significantly inhibit the growth of SGC-7901 gastric cancer mice model tumor tissues and decrease the expression of MMP-9,VEGF and bFGF in blood and cancer tissues.
【作者单位】：重庆医科大学附属第一医院胃肠外科;
【基金】：重庆市渝中区科技计划项目(20160117)
【分类号】：R-332;R735.2

【相似文献】