不同构建方法对肾阳虚大鼠模型的影响
发布时间：2018-10-22 16:36
【摘要】：目的:探讨简便有效的肾阳虚建模方法及对大鼠的影响,为方剂药效学及机制研究奠定基础。方法:用腺嘌呤皮下注射和氢化可的松肌肉注射构建肾阳虚大鼠模型,观察大鼠表现及肾脏睾丸病理变化,测定其脏器指数、自主活动度、游泳时间、血清尿素(SU)、血清肌酐(Cr)、SOD和MDA活力。结果:与空白组相比,两种建模方法均使大鼠自主活动度和游泳时间降低(P0.05),肾脏指数和睾丸指数增高(P0.05);腺嘌呤组大鼠的Cr水平、SOD活力和MDA活力均升高(P0.05);氢化可的松组大鼠的Cr水平和SOD活力降低(P0.05)、MDA活力升高(P0.05);病理变化上,两种建模方法均出现炎性细胞浸润肾间质、睾丸生精小管略萎缩,排列疏松,各级生精细胞排列松散等现象。结论:腺嘌呤与氢化可的松构建肾阳虚模型在虚证表现和肾脏睾丸病变上相似,但腺嘌呤可能是通过损害肾滤过水平、睾丸生理功能等引起Cr、SOD和MDA活力升高,氢化可的松则可能是通过耗散机体能量,减弱机体抵抗能力,影响Cr生成、降低SOD活力和增高MDA活力,具体原因有待进一步探究。
[Abstract]:Objective: to explore a simple and effective modeling method of kidney-yang deficiency and its effect on rats, so as to lay a foundation for the pharmacodynamics and mechanism of prescription. Methods: the rat model of kidney yang deficiency was established by subcutaneous injection of adenine and intramuscular injection of hydrocortisone. Serum urea (SU), serum creatinine (Cr), SOD and MDA activity. Results: compared with the blank group, Both modeling methods decreased autonomous activity and swimming time of rats (P0.05), increased renal index and testicular index (P0.05), increased Cr level, SOD activity and MDA activity of rats in adenine group (P0.05); Cr level and SOD level in hydrocortisone group rats. The activity of), MDA was decreased (P0.05), the activity of), MDA was increased (P0.05), and the pathological changes were observed. The inflammatory cells infiltrated the renal interstitial cells, the testicular seminiferous tubules shrank slightly, the arrangement of spermatogenic cells was loose, and so on. Conclusion: the kidney yang deficiency model constructed by adenine and hydrocortisone is similar in deficiency syndrome and renal testicular lesion, but adenine may cause the increase of Cr,SOD and MDA activity by damaging renal filtration level and testicular physiological function. Hydrocortisone may dissipate body energy, weaken body resistance, affect Cr production, decrease SOD activity and increase MDA activity. The specific reasons need to be further explored.
【作者单位】： 广州中医药大学;广州中医药大学附属宝安中医院;
【基金】：国家自然科学基金资助项目(81102714) 国家级大学生创新训练项目(201410572026) 广东省大学生创新训练项目(201410572077)
【分类号】：R285.5;R-332
本文编号：2287709
[Abstract]:Objective: to explore a simple and effective modeling method of kidney-yang deficiency and its effect on rats, so as to lay a foundation for the pharmacodynamics and mechanism of prescription. Methods: the rat model of kidney yang deficiency was established by subcutaneous injection of adenine and intramuscular injection of hydrocortisone. Serum urea (SU), serum creatinine (Cr), SOD and MDA activity. Results: compared with the blank group, Both modeling methods decreased autonomous activity and swimming time of rats (P0.05), increased renal index and testicular index (P0.05), increased Cr level, SOD activity and MDA activity of rats in adenine group (P0.05); Cr level and SOD level in hydrocortisone group rats. The activity of), MDA was decreased (P0.05), the activity of), MDA was increased (P0.05), and the pathological changes were observed. The inflammatory cells infiltrated the renal interstitial cells, the testicular seminiferous tubules shrank slightly, the arrangement of spermatogenic cells was loose, and so on. Conclusion: the kidney yang deficiency model constructed by adenine and hydrocortisone is similar in deficiency syndrome and renal testicular lesion, but adenine may cause the increase of Cr,SOD and MDA activity by damaging renal filtration level and testicular physiological function. Hydrocortisone may dissipate body energy, weaken body resistance, affect Cr production, decrease SOD activity and increase MDA activity. The specific reasons need to be further explored.
【作者单位】： 广州中医药大学;广州中医药大学附属宝安中医院;
【基金】：国家自然科学基金资助项目(81102714) 国家级大学生创新训练项目(201410572026) 广东省大学生创新训练项目(201410572077)
【分类号】：R285.5;R-332
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1 张翥;马继伟;;溢尿灵对肾阳虚大鼠模型免疫器官的影响[J];天津中医药;2007年01期
，本文编号：2287709
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