菖蒲郁金汤对TS模型大鼠抗抽动效应及脑组织IL-6、TNF-α的影响

发布时间：2018-10-24 13:16

【摘要】：目的:探讨菖蒲郁金汤对多发性抽动症模型大鼠行为及脑组织IL-6及TNF-α的影响。方法:82只雄性大鼠随机分为空白组12只,造模组70只,采用亚氨基二丙腈(IDPN)腹腔注射法制备多发性抽动症动物模型,造模成功后,除去体重偏差较大的10只大鼠,将造模组其余60只大鼠再次分为:模型组、泰必利组、菖蒲郁金汤高、中、低剂量组,各12只。从造模成功后第2天开始给予灌胃,泰必利组给予泰必利(47.91mg/kg),菖蒲郁金汤各剂量组给予菖蒲郁金汤(77.28g/kg、38.64g/kg、19.32g/kg),空白组与模型组给予等体积纯净水,连续给药4周。造模成功后及每周灌胃后1小时,观察大鼠行为。在最后一次给药后24小时计算胸腺指数、脾脏指数,用ELISA法检测IL-6、TNF-α水平;观察纹状体组织病理学变化。结果:1造模后,各组大鼠均出现刻板行为和运动行为;治疗4周后,泰必利组及菖蒲郁金汤组(77.28/kg、38.64g/kg)刻板行为评分明显下降;泰必利组及菖蒲郁金汤组(77.28/kg)运动行为评分明显下降。2模型组大鼠胸腺指数和脾脏指数均明显下降;菖蒲郁金汤高剂量(77.28g/kg)组可明显提高胸腺指数和脾脏指数。3各组大鼠脑组织中IL-6含量无明显变化;模型组大鼠脑组织中TNF-α水平明显下降;泰必利(47.91mg/kg)组大鼠脑组织中TNF-α水平无明显变化;菖蒲郁金汤高剂量(77.28/kg)组大鼠脑组织中TNF-α水平明显升高。4空白组大鼠纹状体神经细胞分布均匀、大小适中、形态如常;模型组大鼠纹状体神经细胞减少,以神经元细胞减少为主,且神经元细胞体积缩小,形态不规则;泰必利(47.91mg/kg)组大鼠纹状体神经细胞数量及形态变化与模型组无明显区别;菖蒲郁金汤各剂量(77.28g/kg、38.64g/kg、19.32g/kg)组纹状体神经元细胞数量及形态均有所改善,以高剂量(77.28g/kg)组为最显著。结论:菖蒲郁金汤具有抗抽动作用,其抗抽动作用可能与改善多发性抽动症模型大鼠免疫功能及修复神经细胞有关。
[Abstract]:Objective: to investigate the effects of Aulupuyujin decoction on behavior and IL-6 and TNF- 伪 in brain tissue of rats with multiple tic syndrome. Methods: 82 male rats were randomly divided into control group (n = 12) and model group (n = 70). The animal model of multiple tic syndrome was established by intraperitoneal injection of iminodipropionitrile (IDPN). The other 60 rats in the model group were divided into three groups: model group, tiapride group, high, middle and low dosage groups, each with 12 rats. From the second day after the model was made, the rats in the tiapride group were given tiapride (47.91mg/kg) and Acorus tujujin decoction (77.28 g / kg ~ (38.64) g / kg ~ (19. 32 g / kg). The blank group and the model group were given the same volume of pure water for 4 weeks. The behavior of rats was observed after successful establishment and 1 hour of gastric perfusion every week. Thymus index and spleen index were calculated 24 hours after the last administration, IL-6,TNF- 伪 levels were detected by ELISA method, and the histopathological changes of striatum were observed. Results: (1) after 4 weeks of treatment, the scores of stereotype behavior and motor behavior were significantly decreased in the Tiapride group and the Acorus Yujin decoction group (77.28% kg / kg 38.64 g / kg). The scores of motor behavior in Tiapride group and Aulunpuyujin decoction group (77.28/kg) were significantly decreased. 2 the thymus index and spleen index in the model group were significantly decreased. The thymus index and spleen index were significantly increased in 77.28g/kg group. 3 there was no significant change in IL-6 content in brain tissue of rats in each group, and the level of TNF- 伪 in brain tissue in model group decreased significantly. The level of TNF- 伪 in the brain tissue of rats in 47.91mg/kg group had no significant change, and that of TNF- 伪 in brain tissue of rats in high dose group (77.28/kg) group was significantly increased. 4 the neurons in striatum of blank group were evenly distributed, moderate in size and normal in shape. The number and morphology of striatal neurons in 47.91mg/kg group were not different from those in model group. The number and morphology of striatum neurons were improved in different doses (77.28g / kg, 38.64g / kg, 19.32g / kg), especially in the group of high dose (77.28g/kg). Conclusion: Liangpu Yujin decoction has antitic effect, which may be related to the improvement of immune function and the repair of nerve cells in rats with multiple tic syndrome.
【作者单位】：甘肃中医药大学;
【基金】：甘肃省中药现代制药工程研究院资助项目,项目编号:YWW-2014005
【分类号】：R285.5;R-332

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