不同时期胚胎组织巢蛋白的表达

发布时间：2018-10-24 18:06

【摘要】：目的：观察肺、肾、食管、胃、小肠、大肠在人胎儿发育过程中的形态学变化,检测巢蛋白在人胎儿不同时期肺、肾、食管、胃、小肠、大肠组织的表达及其变化,并推测巢蛋白在人胎儿发育过程中的作用。方法：取3-8月人胎儿肺、肾、食管、胃、小肠、大肠,常规石蜡切片并将其分为两组,一组作HE染色观察这些组织在发育过程中的形态学变化,一组作免疫组织化学染色,检测巢蛋白在胎儿发育过程这些组织中的表达,根据免疫组化结果进行半定量分析以判断巢蛋白在不同时期胎儿组织的表达变化。结果：巢蛋白在人胎儿发育各个时期的肺、肾、食管、胃、小肠、大肠组织均有表达。巢蛋白在肺组织主要表达在支气管旁、肺间质和微血管以及毛细血管壁上,而在肺导气部管道黏膜上皮没有阳性表达。巢蛋白在肾组织主要表达在肾小球、肾小囊壁层和微血管及毛细血管壁上,在近端小管、远端小管的上皮中没有表达。巢蛋白在消化管主要表达在固有层、黏膜下层和肌层内的微血管和毛细血管壁上巢蛋白在这些组织的表达均随胎龄的增加而减少。结论：巢蛋白在胎儿发育过程的多种组织中均有表达,且表达强度随胎龄的变化而变化,提示巢蛋白可能与胎儿发育有关。另外,巢蛋白在胎儿多种组织的的微血管和毛细血管的管壁上均有表达,且随着胎儿发育,其表达逐渐减少,提示巢蛋白在这些器官组织的血管形成过程中起作用。
[Abstract]:Objective: to observe the morphological changes of lung, kidney, esophagus, stomach, small intestine and large intestine during the development of human fetus, and to detect the expression and changes of nestin in lung, kidney, esophagus, stomach, small intestine and large intestine of human fetus at different stages. The role of nestin in the development of human fetus was speculated. Methods: the human fetal lung, kidney, esophagus, stomach, small intestine, large intestine and normal paraffin sections were divided into two groups: one group was stained with HE to observe the morphological changes of these tissues during development, the other group was stained with immunohistochemistry. The expression of nestin in these tissues during fetal development was detected and semi-quantitative analysis was carried out according to the immunohistochemical results to determine the changes of nestin expression in fetal tissues at different stages. Results: nestin was expressed in lung, kidney, esophagus, stomach, small intestine and large intestine of human fetus. The expression of nestin in lung tissue was mainly near the bronchus, pulmonary interstitial and microvessel and capillary wall, but not in the mucosal epithelium of pulmonary duct. Nestin was mainly expressed in glomeruli, renal capsule wall, microvessel and capillary wall, but not in proximal tubule and distal tubule epithelium. Nestin was mainly expressed in the lamina propria in the digestive tract. The expression of nestin in the wall of microvessels and capillaries in the submucosa and myometrium decreased with the increase of gestational age. Conclusion: nestin is expressed in various tissues during fetal development, and the expression intensity varies with gestational age, suggesting that nestin may be related to fetal development. In addition, nestin was expressed on the walls of microvessels and capillaries in many kinds of fetal tissues, and decreased with the development of fetus, suggesting that nestin plays an important role in the angiogenesis of these organs.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R321

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