人外周血单核细胞迁移分化为角膜上皮样细胞初步研究
发布时间：2018-10-29 09:52
【摘要】：目的：通过对单核迁移细胞行体外迁移,探索外周血单核细胞在新鲜角膜缘干细胞和角膜上皮细胞微环境中诱导下,向角膜上皮细胞跨胚层分化的可能性。 方法：1、体外模拟白细胞迁移过程的模型：细胞培养插入皿上室(8μm孔径)铺上Ⅳ型胶原蛋白后插入六空板内,种上血管内皮细胞。加上单核细胞悬液于上室,此时下室加入含650ng/ml MCP-1的培养基。48小时后,移去细胞培养插入皿,收集下室细胞。为了观察对此模型的影响因素,将实验分为二组：第一组：上室加入含体积分数10%FBS的基础培养基：第二组：上室不加入含FBS的基础培养基。向两组下室分别添加浓度为650ng/mlMCP-1和Ong/ml MCP-1,48小时后,移去插入皿,收集下室细胞。倒置相差显微镜观察迁移后细胞形态,流式细胞术分别检测迁移前后单核细胞表面相对特异性标志：CD14。 2、将收集的迁移细胞置于新鲜角膜缘干细胞和角膜上皮细胞微环境中共培养7d,收集细胞,流式细胞术检测诱导前后细胞表面标志物细胞角蛋白(cytokeratin)、波形蛋白(vimentin)的阳性率。 结果：1、流式细胞仪检测,迁移模型模拟前测得上室CD14单核细胞阳性率为19.40%,迁移模型模拟后测得上室CD14单核细胞阳性率为1.44%。2、流式细胞术检测,微环境诱导前迁移细胞Cytokeratin阳性率为1.64%、Vimentin阳性率为96.29%,微环境诱导后迁移细胞Cytokeratin阳性率为11.30%, Vimentin阳性率为31.55%。 结论：1、体外模拟白细胞迁移过程后,观察到人外周血单核细胞在不加入FBS、Ⅳcollagen浓度为lug、MCP-1浓度为650ng/ml实验条件时贴壁细胞较多。2、通过微环境诱导前后迁移单核细胞CK、Vimentin阳性率的改变初步认为人外周血单核细胞可能具备跨胚层分化的潜能。
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【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R329
本文编号：2297360
[Abstract]:% E7%9B%AE%E7%9A%84%EF%BC%9A%E9%80%9A%E8%BF%87%E5%AF%B9%E5%8D%95%E6%A0%B8%E8%BF%81%E7%A7%BB%E7%BB%86%E8%83 % 9E%E8%A1%8C%E4%BD%93%E5%A4%96%E8%BF%81%E7%A7%BB%2C%E6%8E%A2%E7%B4%A2%E5%A4%96%E5%91%A8%E8%A1%80%E5%8D%95 % E6%A0%B8%E7%BB%86%E8%83%9E%E5%9C%A8%E6%96%B0%E9%B2%9C%E8%A7%92%E8%86%9C%E7%BC%98%E5%B9%B2%E7%BB%86%E8%83 % 9E%E5%92%8C%E8%A7%92%E8%86%9C%E4%B8%8A%E7%9A%AE%E7%BB%86%E8%83%9E%E5%BE%AE%E7%8E%AF%E5%A2%83%E4%B8%AD%E8 % AF%B1%E5%AF%BC%E4%B8%8B%2C%E5%90%91%E8%A7%92%E8%86%9C%E4%B8%8A%E7%9A%AE%E7%BB%86%E8%83%9E%E8%B7%A8%E8%83 % 9A8A82E52E552, E582, E696B9, E6B3%, you have the right side, including the following: 3%. 95EFBCCC2C33E5, including E8BF789, E83B1, and E8BF799, E83B9, F8E799, 839E9, the right side of the side, including E8BF8BF88BF87% and E8BF7BF88BF87%, including E88BF88BF88BF87%, including E8BF7BF88BF87% and E8BF87%, including E8BF7BF88BF87%. 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BC%9A%E4%B8%8A%E5%AE%A4%E4%B8%8D%E5%8A%A0%E5%85%A5%E5%90%ABFBS%E7%9A%84%E5%9F%BA%E7%A1%80%E5%9F%B9%E5%85%BB% E5%9F%BA.%E5%90%91%E4%B8%A4%E7%BB%84%E4%B8%8B%E5%AE%A4%E5%88%86%E5%88%AB%E6%B7%BB%E5%8A%A0%E6%B5%93%E5%BA % A6%E4%B8%BA650ng%2FmlMCP-1%E5%92%8COng%2Fml%20MCP-1%2C48%E5%B0%8F%E6%97%B6%E5%90%8E%2C%E7%A7%BB%E5%8E%BB%E6%8F%92%E5%85%A5 % E79A BF2C2C79A BF2B8E79BF2B888B88E88E88092E88BE4E88BE4E88BE4B6B6B9B9B B8E7A9A9B8E7A7A7A5AE7A5AE7A7A5AE7A5AE7A5AE7A7AE7A% should be the right side of the side of B8B7E7A7A%. 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BA1.44%25.2%2C%E6%B5%81%E5%BC%8F%E7%BB%86%E8%83%9E%E6%9C%AF%E6%A3%80%E6%B5%8B%2C%E5%BE%AE%E7%8E%AF%E5%A2% 83E8E8A8A589 and E78E889, including E78E78E782, E8339ECy880E880B1, and 8DE8E88E589, including E78E889, E787 E887, E784%, B8BA1.64%, BBA1.64%, including BBA1.64%, B8BA1.64%, including BBA1.64%, including BBA1.64%. 252CVimentin E987 E998 E88BEA88E998 E78B680A7 E5908E5908E5908E5908E5908E590%, including E8E5BEA8E828E882, E78BEA8E828E889, including E78E87B1E680A7, including E5908E5908E5908E5908E590%. E8BF1, E8BF7, E7BF7, E7ECytokeratin, E98BF7, E787, E880B7, E7, B7, E7, and E7, 8E%87%E4%B8%BA31.55%25.%0D%0A%0D%0A%0D%0A%09%E7%BB%93%E8%AE%BA%EF%BC%9A1%2C%E4%BD%93%E5%A4%96%E6%A8%A1%E6%8B % 9F%E7%99%BD%E7%BB%86%E8%83%9E%E8%BF%81%E7%A7%BB%E8%BF%87%E7%A8%8B%E5%90%8E%2C%E8%A7%82%E5%AF%9F%E5%88%B0 % E4%BA%E5%A4%96%E5%91%A8%E8%A1%80%E5%8D%95%E6%A0%B8%E7%BB%86%E8%83%9E%E5%9C%A8%E4%B8%8D%E5%8A%A0%E5%85 % A5FBS%2C%E2%85%A3collagen%E6%B5%93%E5%BA%A6%E4%B8%BAlug%2CMCP-1%E6%B5%93%E5%BA%A6%E4%B8%BA650ng%2Fml%E5%AE%9E%E9%AA%8C%E6%9D%A1 % E4%BB%B6%E6%97%B6%E8%B4%E5%A3%81%E7%BB%86%E8%83%9E%E8%BE%83%E5%A4%9A.2%2C%E9%80%9A%E8%BF%87%E5%BE%AE % E7%8E%AF%E5%A2%83%E8%AF%B1%E5%AF%BC%E5%89%8D%E5%90%8E%E8%BF%81%E7%A7%BB%E5%8D%95%E6%A0%B8%E7%BB%86%E8%83 % 9ECK%2CVimentin%E9%98%B3%E6%80%A7%E7%8E%87%E7%9A%84%E6%94%B9%E5%8F%98%E5%88%9D%E6%AD%A5%E8%AE%A4%E4%B8%BA%E4%BA%BA % E5%A4%96%E5%91%A8%E8%A1%80%E5%8D%95%E6%A0%B8%E7%BB%86%E8%83%9E%E5%8F%AF%E8%83%BD%E5%85%B7%E5%A4%87%E8%B7 % A8, including the depth of 833, and the depth of E5B1822E5, and the rest of your arms, you put them in charge and put them around you and put them in the right place, and put them in the right place: E6BD9CE883 BD.
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R329
【参考文献】
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