瓜子金对哮喘模型小鼠气道炎症和气道重塑的治疗作用及机制

发布时间：2018-10-30 20:29

【摘要】：目的瓜子金对哮喘模型小鼠气道炎症和气道重塑的治疗作用及其机制。方法将40只BALB/C雌性小鼠,随机分四个组,分别为空白对照组、支气管哮喘模型组、瓜子金治疗组(0.2g/Kg)、糖皮质激素阳性对照组(0.5mg/Kg)。在末次激发24h后取左肺下叶组织行HE、PAS染色,观察小鼠肺组织形态结构变化。取左肺上叶组织用Western blot法检测NF-kB p65蛋白的表达情况。用酶联免疫吸附法(ELISA)检测小鼠支气管肺泡灌洗液(BALF)中白细胞介素-13(IL-13)、IL-4、IL-5含量;用Diff-Quick染色法观察BALF中细胞总数和各种炎症细胞计数。结果哮喘组与空白对照组比较BALF中细胞总数、细胞分类计数、IL-13、IL-5、IL-4的含量增高,肺组织细胞核内NF-kB p65蛋白含量表达明显升高而在细胞质内NF-kB p65蛋白含量则明显降低;而瓜子金治疗组、糖皮质激素组与哮喘模型组相比BALF中细胞总数、各个细胞分类计数、IL-13、IL-5、IL-4的含量明显降低,肺组织细胞核内NF-kB p65蛋白含量表达明显降低而在细胞质内NF-kB p65蛋白含量则明显升高。哮喘组与空白对照组比较,肺组织可见多种炎症细胞浸润,支气管腔内分泌物增多,杯状细胞增殖,气道平滑肌肌层及基底膜增厚,气道上皮细胞下纤维化等气道重塑改变;而瓜子金治疗组、糖皮质激素阳性对照组与哮喘组比较上述组织病理改变均有明显好转。结论瓜子金可能通过抑制Th2类细胞因子及NF-kB p65蛋白表达,而抑制气道炎症和气道重塑的发生、发展。
[Abstract]:Objective to investigate the therapeutic effect and mechanism of Guizeijin on airway inflammation and airway remodeling in asthmatic mice. Methods Forty BALB/C female mice were randomly divided into four groups: blank control group, bronchial asthma model group, Guizejin treatment group (0.2g/Kg) and glucocorticoid positive control group (0.5mg/Kg). HE,PAS staining was performed on the left lower lobe of the lung 24 hours after the last challenge, and the morphologic changes of the mouse lung were observed. The expression of NF-kB p65 protein was detected by Western blot method in the upper lobe of left lung. The contents of interleukin-13 (IL-13) and IL-4,IL-5 in (BALF) of mouse bronchoalveolar lavage fluid (BALF) were detected by (ELISA), and the total number of cells in BALF and the number of inflammatory cells in BALF were observed by Diff-Quick staining. Results compared with the control group, the total number of cells, the cell classification count and the content of IL-13,IL-5,IL-4 in the asthma group were higher than those in the control group. The expression of NF-kB p65 protein increased significantly in the nucleus of lung tissue, but decreased significantly in the cytoplasm of the lung tissue. However, compared with asthma model group, the total number of cells in BALF, the number of cell classification and the content of IL-13,IL-5,IL-4 were significantly decreased in Guizejin group and glucocorticoid group. The expression of NF-kB p65 protein decreased significantly in the nucleus of lung tissue, but increased significantly in the cytoplasm. Compared with the blank control group, there were many inflammatory cells infiltrating in lung tissue, more secretions in bronchial cavity, proliferation of goblet cells, thickening of muscle layer and basement membrane of airway smooth muscle, fibrosis of airway epithelial cells, and other changes of airway remodeling. The histopathological changes of Guizejin treatment group, glucocorticoid positive control group and asthma group were improved obviously. Conclusion Guizejin may inhibit the development of airway inflammation and airway remodeling by inhibiting the expression of Th2 cytokines and NF-kB p65 protein.
【作者单位】：延边大学附属医院;延边大学医学院;
【分类号】：R285.5;R-332

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