裸小鼠淋巴结的解剖和组织学特点

发布时间：2018-10-31 07:08

【摘要】：目的:研究裸小鼠淋巴结的解剖学和组织学特点。方法:使用手术显微镜解剖20只BALB/c裸小鼠和20只BALB/c小鼠,通过免疫组织化学方法比较了两者淋巴结中T淋巴细胞和B淋巴细胞表达的不同。结果:平均每只裸小鼠中包含23个淋巴结和一组大型淋巴结群。裸小鼠颈部淋巴结分布密度较高,其中颌下淋巴结及颈浅淋巴结在裸小鼠中大多数为2个(构成比分别为95%和90%),而在小鼠中大多数为4个(构成比分别为95%和90%),两者差异有统计学意义。无论小鼠还是裸小鼠的颈深淋巴结均为2个(构成比分别为100%和95%),两者差异无统计学意义。裸小鼠淋巴结中CD3阳性的T淋巴细胞减少。细胞膜和细胞质着色的CD3阳性表达的T细胞在裸小鼠的表达阳性率为15%,在小鼠的表达阳性率为100%,两者表达的差异有统计学意义。细胞膜阳性表达CD20的B细胞在裸小鼠中的阳性率为95%,在小鼠中的阳性率为100%,两者表达的差异无统计学意义。结论:裸小鼠淋巴结分布的解剖图对于初学者有一定的帮助,裸小鼠颈部淋巴结密度较高的解剖学特点和缺乏CD3阳性的T淋巴细胞将有助于更好地理解裸小鼠的淋巴结转移机制。
[Abstract]:Objective: to study the anatomical and histological characteristics of lymph nodes in nude mice. Methods: twenty BALB/c nude mice and 20 BALB/c mice were dissected by operation microscope. The expression of T lymphocytes and B lymphocytes in lymph nodes were compared by immunohistochemical method. Results: an average of 23 lymph nodes and a large group of lymph nodes were found in each nude mouse. The distribution density of cervical lymph nodes in nude mice was high. The submaxillary lymph nodes and superficial cervical lymph nodes were mostly 2 (95% and 90%) in nude mice. But in most of the mice, there were 4 (95% and 90%), the difference was statistically significant. There were 2 deep cervical lymph nodes in both mice and nude mice (composition ratios were 100% and 95%, respectively), and there was no significant difference between them. The number of CD3 positive T lymphocytes in lymph nodes of nude mice was decreased. The positive rate of CD3 positive expression of T cells in nude mice was 15%, and that in mice was 100%. The difference between them was statistically significant. The positive rate of B cells expressing CD20 in nude mice was 95%, and that in mice was 100%. There was no significant difference between them. Conclusion: the anatomy of lymph node distribution in nude mice is helpful for beginners. The anatomical characteristics of cervical lymph node density in nude mice and the absence of CD3 positive T lymphocytes will contribute to a better understanding of the mechanism of lymph node metastasis in nude mice.
【作者单位】：山西省人民医院口腔颌面外科;山西医科大学口腔医院口腔颌面外科;北京大学口腔医学院·口腔医院口腔颌面外科口腔数字化医疗技术和材料国家工程实验室口腔数字医学北京市重点实验室;
【基金】：国家自然科学基金(30872899) 山西省自然科学基金(2011011037)资助~~
【分类号】：R-332

【相似文献】