人胎盘间充质样干细胞的分离、培养及其生物学特性分析

发布时间：2018-11-01 15:28

【摘要】：从组织块中分离纯化成体干细胞具有一定的困难,其他细胞的混杂成为获取高纯度组织干细胞的一个技术瓶颈.以人体胎盘为研究对象,在分散组织细胞的基础上,通过不同时间梯度贴壁方法分离纯化了人胎盘间充质样干细胞,并与常规的直接贴壁方法作了比较,同时分析了时间梯度贴壁方法分选细胞的增殖能力、多分化潜能以及免疫原性.研究结果表明,时间梯度贴壁法获得的细胞具有较强的增殖能力.直接贴壁法得到的细胞不具有分化潜能,而时间梯度贴壁法获得的细胞具有向成骨细胞和成脂肪细胞方向分化的能力.通过对免疫原性基因检测的分析,表明时间梯度贴壁法获得的细胞表达HLA-Ⅰ,但不表达HLA-Ⅱ.
[Abstract]:It is difficult to isolate and purify adult stem cells from tissue blocks, and the mixing of other cells has become a technical bottleneck in obtaining high purity tissue stem cells. Human placenta mesenchymal stem cells were isolated and purified by different time gradient adherent methods on the basis of dispersed tissue cells. At the same time, the proliferative ability, multi-differentiation potential and immunogenicity of the cells were analyzed by time gradient adherent method. The results showed that the cells obtained by time gradient adhesion method had strong proliferative ability. The cells obtained by direct adherent method have no differentiation potential, but the cells obtained by time gradient adhesion method have the ability to differentiate into osteoblasts and adipoblasts. The analysis of immunogenicity gene showed that the cells obtained by time gradient adhesion method expressed HLA- 鈪，

本文编号：2304330