手足综合征大鼠模型血浆细胞因子的变化

发布时间：2018-11-01 15:16

【摘要】：目的建立大鼠手足综合征模型,探讨手足综合征发病过程中相关炎性因子的水平及发病机制。方法运用卡培他滨制作手足综合征大鼠模型,分为给药1周期的A组和给药两周期的B组(连续给药7d,间隔3d,10d为一个周期),分别模拟不同给药剂量下发生的手足综合征,留取大鼠足跖部图像资料及血浆标本,检测血浆中亲胆碱能神经元因子(CNTF)、血小板衍生的内皮细胞生长因子(PD-ECGF)、白介素β(IL-β)、白介素10(IL-10)、中性粒细胞趋化因子(CINC-3)水平。结果与正常大鼠相比,1周期模型大鼠PD-ECGF,CINC-3,CNTF,IL-10,IL-β水平均升高(P0.01);而1周期造模未成功大鼠、模型大鼠停药1周后各细胞因子水平与正常大鼠相比的差异无统计学意义(P0.05)。与正常大鼠相比,2周期模型大鼠及模型大鼠停药1周后CINC-3,CNTF水平明显升高(P0.01);而余各因子在各组间水平差异无统计学意义(P0.05)。结论在短期给药过程中,HFS的发生主要与广泛发生的炎性损伤有关,而在较长时间的给药后,广泛的炎性反应趋于控制,而主要反应为相关炎性细胞的趋化及神经元的损伤。
[Abstract]:Objective to establish a rat model of hand and foot syndrome and to explore the level and pathogenesis of inflammatory factors in the pathogenesis of hand and foot syndrome. Methods the rat model of hand and foot syndrome was established by capecitabine. The rats were divided into two groups: group A (1 cycle) and group B (2 cycles) (7 days of continuous administration of capecitabine at intervals of 3 days and 10 days as a cycle). The paw and metatarsal images of rats and plasma samples were used to detect the endothelium-derived endothelial growth factor (PD-ECGF) derived from (CNTF), platelet-derived cholinergic neuron factor (CNTF),). Levels of interleukin 尾 (IL- 尾), interleukin 10 (IL-10) and neutrophil chemokine (CINC-3). Results compared with normal rats, the level of PD-ECGF,CINC-3,CNTF,IL-10,IL- 尾 increased in 1 cycle model rats (P0.01). However, the level of cytokines in the model rats was not significantly different from that in the normal rats after 1 week of withdrawal (P0.05). Compared with normal rats, the level of CINC-3,CNTF increased significantly in 2-cycle model rats and model rats after 1 week of withdrawal (P0.01), while the other factors were not significantly different among the groups (P0.05). Conclusion during the short-term administration of HFS, the occurrence of HFS is mainly related to the extensive inflammatory injury, and the extensive inflammatory response tends to be controlled after a long time of administration, and the main response is the chemotaxis of the related inflammatory cells and the injury of neurons.
【作者单位】：河北北方学院研究生院;解放军第251医院;河北北方学院;
【基金】：全军“十二五”中医药重点项目(10ZYZ105) 河北省研究生创新资助项目(285)
【分类号】：R-332;R730.53

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