益气健脾方对脾气虚证模型大鼠心肌细胞线粒体呼吸链酶复合物活性的影响
发布时间：2018-11-02 14:41
【摘要】：目的探究益气健脾方对脾气虚证模型大鼠心肌细胞线粒体呼吸链酶复合物活性的影响。方法将30只SD大鼠随机分为正常组、模型组、治疗组,每组10只,对3组大鼠进行分笼饲养。模型组和治疗组大鼠采取饮食干预联合极限游泳法进行脾气虚造模,于实验第15天对3组大鼠的体质量、肛温、食量、饮水量及脾气虚症状积分进行测评。造模成功后,正常组和模型组采用常规饲养方式联合生理盐水灌胃,治疗组采用常规饲养方式联合益气健脾中药汤剂灌胃,时间为8周。之后分别取3组大鼠的心肌线粒体,测定心肌组织线粒体呼吸链酶复合物活性。结果模型组大鼠的线粒体呼吸链酶复合物Ⅱ和复合物Ⅳ活性明显低于正常组(P0.05);治疗组大鼠的线粒体呼吸链酶复合物Ⅱ和复合物Ⅳ活性与模型组比较差异具有统计学意义(P0.05)。结论脾气虚能够造成心肌细胞线粒体发生突变和缺失,导致呼吸链酶复合物活性下降,益气健脾中药能够调节心肌组织线粒体呼吸链酶复合物Ⅱ和复合物Ⅳ活性。
[Abstract]:Objective to investigate the effect of Yiqi Jianpi recipe on the activity of mitochondrial respiratory chain enzyme complex in cardiac myocytes of rats with deficiency of qi. Methods Thirty SD rats were randomly divided into normal group, model group and treatment group. The rats in the model group and the treatment group were treated with diet intervention combined with limited swimming method to make models of spleen qi deficiency. On the 15th day of the experiment, the body weight, anus temperature, food quantity, drinking water and the integral score of spleen qi deficiency symptom of the three groups were measured. After the model was successfully made, the normal and model groups were fed with normal feeding method combined with normal saline, and the treatment group were treated with routine feeding method combined with Yiqi Jianpi traditional Chinese medicine decoction for 8 weeks. Myocardial mitochondria were taken from three groups of rats, and the activity of respiratory chain enzyme complex in myocardial mitochondria was measured. Results the activities of mitochondrial respiratory chain enzyme complex 鈪，
本文编号：2306094
[Abstract]:Objective to investigate the effect of Yiqi Jianpi recipe on the activity of mitochondrial respiratory chain enzyme complex in cardiac myocytes of rats with deficiency of qi. Methods Thirty SD rats were randomly divided into normal group, model group and treatment group. The rats in the model group and the treatment group were treated with diet intervention combined with limited swimming method to make models of spleen qi deficiency. On the 15th day of the experiment, the body weight, anus temperature, food quantity, drinking water and the integral score of spleen qi deficiency symptom of the three groups were measured. After the model was successfully made, the normal and model groups were fed with normal feeding method combined with normal saline, and the treatment group were treated with routine feeding method combined with Yiqi Jianpi traditional Chinese medicine decoction for 8 weeks. Myocardial mitochondria were taken from three groups of rats, and the activity of respiratory chain enzyme complex in myocardial mitochondria was measured. Results the activities of mitochondrial respiratory chain enzyme complex 鈪，
本文编号：2306094
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