人类脐带血MSC和骨髓MSC分泌细胞因子的对比研究
发布时间：2018-11-04 12:39
【摘要】：目的应用人类脐带血和健康人骨髓这两种材料,分别培养出间充质干细胞,然后比较两种细胞分泌支持造血调控细胞因子的差异,为体外高效造血干细胞扩增体系的确立提供参考,明确脐带血来源的间充质干细胞的支持造血的能力。 方法用ELISA方法,测定两种细胞分泌的干细胞生长因子(Stem cell factor , SCF),血小板生成素(Thrombopoietin , TPO)和白细胞介素6(Interleukin-6,IL-6)的浓度,并且进行对比。 结果在体外培养过程中,脐带血及骨髓中的间充质干细胞均可表达干细胞生长因子(Stem cell factor , SCF),血小板生成素(Thrombopoietin , TPO)和白细胞介素6(Interleukin-6,IL-6)。但是相同生长条件下,脐带血及骨髓中的间充质干细胞分泌的因子浓度不同。人脐血基质细胞分泌SCF的量(575.7±40.2pg/ml)高于骨髓基质细胞(482.2±17.7pg/ml),而人脐血基质细胞分泌IL-6 (469.7±27.2pg/ml)的量低于同期培养的骨髓基质细胞(935.19±35.4pg/ml),人脐血基质细胞TPO分泌的量(195.5±7.3pg/ml)与骨髓基质细胞(172.2±9.3pg/ml)相近。
[Abstract]:Objective to culture mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB) and healthy human bone marrow (BMSCs), and compare the difference of cytokines secreted by two kinds of cells in supporting hematopoietic regulation. To provide a reference for the establishment of an in vitro high efficient hematopoietic stem cell amplification system and to clarify the ability of umbilical cord blood derived mesenchymal stem cells to support hematopoiesis. Methods ELISA assay was used to detect the concentrations of (Stem cell factor, SCF), thrombopoietin (Thrombopoietin, TPO) and interleukin 6 (Interleukin-6,IL-6) secreted by two kinds of stem cell growth factor (Stem cell factor, SCF), and the results were compared. Results in vitro, mesenchymal stem cells in cord blood and bone marrow could express stem cell growth factor (Stem cell factor, SCF), thrombopoietin (Thrombopoietin, TPO) and interleukin 6 (Interleukin-6,IL-6). However, under the same growth conditions, the concentration of factors secreted by mesenchymal stem cells in cord blood and bone marrow varied. The amount of SCF secreted by human umbilical cord blood stromal cells (575.7 卤40.2pg/ml) was higher than that of bone marrow stromal cells (482.2 卤17.7pg/ml). However, the amount of IL-6 secreted by human umbilical cord blood stromal cells (469.7 卤27.2pg/ml) was lower than that of bone marrow stromal cells cultured at the same time (935.19 卤35.4pg/ml). The amount of TPO secreted by human umbilical cord blood stromal cells (195.5 卤7.3pg/ml) was similar to that of bone marrow stromal cells (172.2 卤9.3pg/ml).
【学位授予单位】：广州医学院
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R329
本文编号：2309839
[Abstract]:Objective to culture mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB) and healthy human bone marrow (BMSCs), and compare the difference of cytokines secreted by two kinds of cells in supporting hematopoietic regulation. To provide a reference for the establishment of an in vitro high efficient hematopoietic stem cell amplification system and to clarify the ability of umbilical cord blood derived mesenchymal stem cells to support hematopoiesis. Methods ELISA assay was used to detect the concentrations of (Stem cell factor, SCF), thrombopoietin (Thrombopoietin, TPO) and interleukin 6 (Interleukin-6,IL-6) secreted by two kinds of stem cell growth factor (Stem cell factor, SCF), and the results were compared. Results in vitro, mesenchymal stem cells in cord blood and bone marrow could express stem cell growth factor (Stem cell factor, SCF), thrombopoietin (Thrombopoietin, TPO) and interleukin 6 (Interleukin-6,IL-6). However, under the same growth conditions, the concentration of factors secreted by mesenchymal stem cells in cord blood and bone marrow varied. The amount of SCF secreted by human umbilical cord blood stromal cells (575.7 卤40.2pg/ml) was higher than that of bone marrow stromal cells (482.2 卤17.7pg/ml). However, the amount of IL-6 secreted by human umbilical cord blood stromal cells (469.7 卤27.2pg/ml) was lower than that of bone marrow stromal cells cultured at the same time (935.19 卤35.4pg/ml). The amount of TPO secreted by human umbilical cord blood stromal cells (195.5 卤7.3pg/ml) was similar to that of bone marrow stromal cells (172.2 卤9.3pg/ml).
【学位授予单位】：广州医学院
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R329
【参考文献】
相关期刊论文 前2条
1 谢小燕,谢超,李锦,白慈贤,裴雪涛;具有维持造血干/祖细胞能力的新型豆类凝集素的分离、纯化及功能鉴定[J];生物化学与生物物理进展;2004年02期
2 夏文杰;许茹;叶欣;付涌水;罗广平;丁浩强;项鹏;张秀明;邓晶;陈扬凯;;人骨髓间充质干细胞体外扩增的生物学评估[J];中国实验血液学杂志;2008年03期
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