小鼠急性肺损伤造模条件的探究

发布时间：2018-11-06 09:27

【摘要】：为探究构建急性肺损伤模型的条件,本试验选用健康昆明小鼠,经腹腔注射递增剂量脂多糖(LPS)(0、2、4、6、8、10mg/kg),观察不同条件下小鼠呼吸频率、死亡率、肺脏干湿重比值及组织结构变化,检测炎症因子IL-10、TNF-α、IL-1β及IFN-γ在不同程度肺损伤中的表达量变化。结果显示,4mg/kg LPS组肺脏干湿重比值显著高于对照组(P0.05);6、8和10m/kg LPS组肺脏干湿重比值极显著高于对照组(P0.01)。病理切片分析显示,6mg/kg LPS组小鼠肺脏出现充血,肺泡腔及肺间质出现渗出,肺泡隔增厚,出现少量中性粒细胞浸润;8、10mg/kg LPS组小鼠肺脏充血明显,肺泡腔及肺间质出现渗出,肺泡明显隔增厚,出现中性粒细胞浸润;与对照组相比,6、8、10mg/kg LPS组肺组织中胶原纤维大量增多,且多出现在肺气管周围,10mg/kg LPS组现象最明显。炎症因子检测分析结果显示,与对照组相比,2、4、6、8、10mg/kg LPS组炎症因子均极显著增加(P0.01)。结合病理组织切片及相关检测指标表明,昆明小鼠腹腔注射8mg/kg LPS并作用12h,可成功构建急性肺损伤模型。
[Abstract]:In order to explore the conditions for establishing acute lung injury model, healthy Kunming mice were used to observe the respiratory rate and mortality rate of mice under different conditions by intraperitoneal injection of lipopolysaccharide (LPS) (). The changes of lung dry-wet weight ratio and tissue structure, the expression of inflammatory factors IL-10,TNF- 伪, IL-1 尾 and IFN- 纬 in different degree of lung injury were detected. The results showed that the ratio of lung dry-wet weight in 4mg/kg LPS group was significantly higher than that in control group (P0.05), and the ratio of lung dry-wet weight in 6F8 and 10m/kg LPS group was significantly higher than that in control group (P0.01). Pathological analysis showed that in 6mg/kg LPS group, hyperemia appeared in lung, exudation in alveolar cavity and interstitium, thickening of alveolar septum and infiltration of a few neutrophils. The lung congestion, alveolar cavity and interstitial exudation, alveolar thickening and neutrophil infiltration were observed in 810 mg / kg LPS group. Compared with the control group, the collagen fibers in lung tissue in the group of 6o 8mg / kg LPS increased significantly, and most of them appeared around the pulmonary duct. The phenomenon of 10mg/kg LPS group was the most obvious. Compared with the control group, the inflammatory factors were significantly increased in the 10 mg / kg LPS group (P0.01). In combination with pathological sections and related detection indexes, Kunming mice were injected intraperitoneally with 8mg/kg LPS for 12 h to successfully construct acute lung injury model.
【作者单位】：华中农业大学动物科学技术学院/动物医学院;
【基金】：国家自然科学基金青年基金项目(31101776) 华中农业大学自主科技创新项目(2662015PY063)
【分类号】：R-332;R563

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