红芪等4种中药多糖对环磷酰胺所致免疫低下小鼠模型的免疫调节作用对比研究

发布时间：2018-11-14 16:42

【摘要】：目的对比研究红芪等4种中药多糖对环磷酰胺所致免疫低下小鼠模型的免疫调节作用。方法用环磷酰胺40 mg·kg~(-1)制备免疫低下小鼠模型。按照体重将昆明种小鼠随机分为7组:正常组、模型组、对照组、红芪多糖组、黄芪多糖组、党参多糖组、当归多糖组。对照组灌胃参芪颗粒5 g·kg~(-1),其他各组分别灌胃相应的受试样品2 g·kg~(-1),正常组和模型组灌胃等量0.9%NaCl,每天给药1次,连续15 d。称重法计算小鼠脏器指数;腹腔注射5%鸡红细胞悬液测定腹腔巨噬细胞吞噬百分率及吞噬指数;50%二硝基氯苯丙酮溶液致迟发型超敏反应测定OD值;5%鸡红细胞悬液(CRBC)致敏测定血清溶血素OD值;尾静脉注射印度墨汁测定网状内皮系统吞噬指数及吞噬系数;用紫外分光光度法测定多糖含量。结果与正常组比较,模型组各指标差异均有统计学意义(均P0.01)。给药后,模型组与红芪多糖组、黄芪多糖组、党参多糖组、当归多糖组的胸腺指数(g·kg~(-1))分别是2.55±0.88,1.04±0.74,2.00±0.89,2.21±0.95,1.92±0.43,1.67±0.41,与模型组比较,红芪多糖组、黄芪多糖组、党参多糖组差异均有统计学意义(均P0.05)。这6组的腹腔巨噬细胞吞噬指数分别是1.31±0.18,0.51±0.12,1.12±0.13,1.19±0.09,0.88±0.25,0.72±0.07,与模型组比较,4种多糖组差异均有统计学意义(均P0.01);与当归多糖组比较,红芪多糖组、黄芪多糖组的差异均有统计学意义(P0.05或P0.01)。这6组的网状内皮系统吞噬系数分别是6.60±0.94,0.99±0.83,0.32±1.48,7.28±2.13,5.29±1.97,5.93±1.75,与模型组比较,红芪多糖组、黄芪多糖组差异均有统计学意义(P0.05或P0.01)。这6组的迟发型超敏反应OD值分别为0.53±0.16,0.23±0.09,0.36±0.09,0.38±0.12,0.29±0.10,0.33±0.11;这6组的血清溶血素OD值分别为0.60±0.12,0.43±0.10,0.53±0.08,0.54±0.11,0.51±0.11,0.52±0.09;与模型组比较,红芪多糖、黄芪多糖、当归多糖迟发型超敏反应OD值和血清溶血素OD值差异均有统计学意义(P0.05或P0.01)。结论这4种中药多糖均具有提高小鼠非特异性免疫功能、特异性体液免疫功能和细胞免疫功能的作用。但红芪多糖与黄芪多糖对各免疫指标的调节要更全面且显著一些。
[Abstract]:Objective to study the immunomodulatory effects of four traditional Chinese medicine polysaccharides such as Hongqi on immunosuppression mice induced by cyclophosphamide (cyclophosphamide). Methods the immunocompromised mouse model was established with cyclophosphamide 40 mg kg~ (-1). According to body weight, Kunming mice were randomly divided into 7 groups: normal group, model group, control group, red astragalus polysaccharide group, Codonopsis polysaccharide group, angelica sinensis polysaccharide group. The control group was given Shenqi granule 5 g kg~ (-1), the other groups were given 2 g kg~ (-1) respectively. The normal group and the model group were given the same amount of 0.9 NaCl2, once a day for 15 days. The abdominal macrophage phagocytosis percentage and phagocytic index were measured by intraperitoneal injection of 5% chicken red blood cell suspension and the OD value by 50% dinitrochlorophenone solution induced delayed type hypersensitivity reaction. Serum hemolysin OD was determined by sensitizing 5% chicken red blood cell suspension (CRBC), phagocytosis index and phagocytic coefficient of reticular endothelial system were determined by Indian ink injection via tail vein, and polysaccharide content was determined by ultraviolet spectrophotometry. Results compared with the normal group, there were significant differences between the model group and the normal group (P 0.01). After administration, the thymus index (g kg~ (-1) of the model group, the Hongqi polysaccharide group, the astragalus polysaccharide group, the Codonopsis polysaccharide group and the Angelica polysaccharide group were 2.55 卤0.888U 1.04 卤0.742.00 卤0.89U 2.21 卤0.95U 1.92 卤0.43n 1.67 卤0.41, respectively. Compared with the model group, there were significant differences among the red astragalus polysaccharide group, the astragalus polysaccharide group and the Codonopsis pilosula polysaccharide group (P0.05). The phagocytic index of peritoneal macrophages in these six groups was 1.31 卤0.18g 0.51 卤0.12g 1.12 卤0.131.19 卤0.09U 0.88 卤0.25U 0.72 卤0.07respectively. Compared with the model group, there were significant differences among the four polysaccharides groups (all P0.01). Compared with Angelica polysaccharide group, there were significant differences between Hongqi polysaccharide group and astragalus polysaccharide group (P0.05 or P0.01). The phagocytic coefficient of reticular endothelial system in these six groups was 6.60 卤0.94U 0.99 卤0.83U 0.32 卤1.48U 7.28 卤2.137N 5.29 卤1.97 卤5.93 卤1.75, respectively. Astragalus polysaccharide group differences were statistically significant (P0.05 or P0.01). The OD values of delayed type hypersensitivity in the 6 groups were 0.53 卤0.160.23 卤0.09U 0.36 卤0.09U 0.38 卤0.120.29 卤0.10g 0.33 卤0.11, respectively. The OD values of serum hemolysin in these 6 groups were 0.60 卤0.12 卤0.43 卤0.10 ~ 0.53 卤0.08 卤0.54 卤0.11 ~ 0.51 卤0.11 ~ 0.52 卤0.09, respectively. Compared with the model group, there were significant differences in OD value and serum hemolysin OD value (P0.05 or P0.01) among the three groups. Conclusion these four kinds of polysaccharides can improve the function of nonspecific immunity, specific humoral immunity and cellular immunity in mice. However, the regulation of Astragalus polysaccharides and Astragalus polysaccharides on each immune index is more comprehensive and more significant.
【作者单位】：甘肃中医药大学药学院;甘肃中医药大学甘肃省高校中(藏)药化学与质量研究省级重点实验室;甘肃中医药大学甘肃省中药药理与毒理学重点实验室;
【基金】：甘肃省自然科学基金资助项目(17JR5RA163) 甘肃省高等学校基本科研业务费基金资助项目(2013-1) 甘肃省高校中(藏)药化学与质量研究省级重点实验室开放基金资助项目(zzy-2014-04) 甘肃省教育厅科研基金资助项目(2013A-085)
【分类号】：R285.5;R-332

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