人乳头瘤病毒58型L1蛋白B细胞表位预测和分析

发布时间：2018-11-14 20:04

【摘要】：目的对人乳头瘤病毒58型(HPV58)主要结构蛋白(L1)的B细胞表位进行预测和分析。方法利用生物信息学软件EXPASY在线分析HPV58型L1蛋白的二级结构及生物学特性,包括跨膜趋势、表面可及性、抗原性、亲水性,溶性等,从而对其B细胞表位进行综合分析和预测。结果通过亲水性参数,Zimmcrman极性,柔韧性参数,表面可及性及抗原性进行综合分析,预测人乳头瘤病毒58型L1蛋白的B细胞表位可能位于其N端的29-37,43,46-58,64-67,74,79-88,93,100-101,103-125,133-139,149,151-180,187-213,220-213,235,238-247,252-271,277-282,286-309,320-323,327-338,341-348,351,353-368,373-396,408,427,419-425,433-444,453-468,470,475-486,488-490,493-494,497-524肽段区域;进一步用Protien Plast在线同源匹配分析,79-88、190-216、373-396、453-468、497-524肽段区段为可能的B细胞表位。结论用多参数预测分析HPV58型L1蛋白可能的B细胞表位,为进一步研究高危型58型L1蛋白的生物学特性提供了理论基础。
[Abstract]:Objective to predict and analyze B cell epitopes of the main structural protein (L1) of human papillomavirus 58 (HPV58). Methods the secondary structure and biological characteristics of HPV58 L1 protein were analyzed online by bioinformatics software EXPASY, including the transmembrane trend, surface accessibility, antigenicity, hydrophilicity, solubility and so on. The B cell epitopes were comprehensively analyzed and predicted. Results the hydrophilic parameters, Zimmcrman polarity, flexibility parameters, surface accessibility and antigenicity were comprehensively analyzed. It is predicted that the B cell epitope of human papillomavirus 58 L1 protein may be located in its N-terminal of 29-37-43-36-58-43-67-77-79-8A1-93100-101103-125133-139149151-180187-213220-213235238-247252-271277-282286-302286-323327-338341-348351351-368373-39640842719-425433-444453 -468470475-486488-490493-494497-524 peptide region; Further more, 79-88190-216373-396453-468497-524 peptide region was identified as a possible B cell epitope by Protien Plast online homologous matching analysis. Conclusion the multiparameter prediction and analysis of the possible B cell epitopes of HPV58 L1 protein may provide a theoretical basis for the further study of the biological characteristics of high risk type 58 L1 protein.
【作者单位】：温州医科大学微生物学与免疫学教研室;
【基金】：国家自然科学基金项目(No.81176423)
【分类号】：R373

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