血管内皮生长因子基因转染的人脂肪干细胞具有较强的增殖和分化能力

发布时间：2018-11-15 16:39

【摘要】：目的研究过表达血管内皮生长因子(VEGF)对人脂肪干细胞(hADSC)的细胞表型、增殖能力和多向分化潜能的影响。方法利用脂质体法将pIRES2-EGFP-VEGF质粒转染第2代hADSC,对照组为脂质体空载体。免疫荧光染色验证转染成功,流式细胞术检测两组细胞表型的差异,MTT法检测细胞增殖能力,诱导其成脂和成骨分化并进行油红O和茜素红染色。结果转染组可见EGFP和VEGF的表达,对照组无EGFP表达。两组细胞均高表达CD29、CD44、CD90,低表达CD31、CD45。转染组hADSC的增殖能力显著强于对照组,且诱导分化后的脂滴和钙结节的数量和面积均显著高于对照组。结论过表达VEGF可增强hADSC的增殖能力,促进其成脂和成骨分化,但并未显著改变细胞表型。
[Abstract]:Objective to study the effects of overexpression of vascular endothelial growth factor (VEGF) on the phenotype, proliferative ability and multipotential differentiation of human adipose stem cells (hADSC). Methods pIRES2-EGFP-VEGF plasmid was transfected into the second generation hADSC, control group by liposome method. The transfection was successfully verified by immunofluorescence staining. The phenotypic differences between the two groups were detected by flow cytometry. The proliferative ability of the cells was detected by MTT method and the adipogenic and osteogenic differentiation was induced and stained with oil red O and alizarin red. Results the expression of EGFP and VEGF was found in the transfection group, but no EGFP expression was found in the control group. High expression of CD29,CD44,CD90, and low expression of CD31,CD45. in both groups The proliferative ability of hADSC in transfection group was significantly stronger than that in control group, and the number and area of lipid droplets and calcium nodules after induced differentiation were significantly higher than those in control group. Conclusion overexpression of VEGF can enhance the proliferation of hADSC and promote its adipogenic and osteogenic differentiation, but it does not significantly change the phenotype of hADSC.
【作者单位】：解放军总医院整形修复科;美国德克萨斯大学MD安德森肿瘤中心整形外科;美国贝勒医学院医学系肾脏学部;
【基金】：国家自然科学基金(81272123,81571864)
【分类号】：R329.2

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