建立大鼠胆管良性狭窄模型的实验研究

发布时间：2018-11-15 20:03

【摘要】：目的：探讨建立简捷、经济实用、稳定可靠的大鼠胆管良性狭窄动物模型的方法,利于对胆管良性狭窄的发生机制和有效治疗方法的研究。方法： 49只雌性SD (Sprague Dawley)大鼠体重220-260克,平均240±10 g,随机将其分为实验组21只、实验对照组21只和空白对照组7只,所有实验动物均采用3%的戊巴比妥注射液进行腹腔麻醉。实验组21只动物进行腹腔麻醉后上腹剑突下正中切口开腹,显露胆总管后在距离十二指肠上缘2cm处分离出胆总管长约1cm,其下面放置一厚约0.2cm的棉垫后滴注95%的酒精约0.7ml于胆管上持续2-3分钟后关腹；实验对照组以同样的方法进行手术,在胆管上滴注0.9%的生理盐水后2-3分钟关腹；空白对照组只是找出胆管后就关腹,不进行其他处理。术后观察包括精神状态、尿液颜色、进食情况;实验模型组和实验对照组术后7、14、21天共分三次每次分别取7只动物称体重与及时抽血检查肝功能(ALT、GGT、T-BIL、D-BIL),并切取部分肝脏和实验处胆管行病理学检查,21天组还进行胆道造影了解胆管梗阻情况。统计学采用SPSS软件t检验及多元方差分析。结果：术后1hSD大鼠能翻身活动,3-4h后自主饮水,逐渐恢复饮食。①从术前到术后21天,实验造模组检测血清丙氨酸氨基转移酶(ALT)、γ-谷氨酰转移酶(γ-glutamyl transferase,GGT)、血清总胆红素(T-BIL)、血清直接胆红素(D-BIL)等指标有明显变化(P0.05)；而对照组和空白对照组无显著变化。②各时间点处死大鼠切取肝脏及胆管行病理学检查,实验造模组随着时间推移发现肝细胞有不同程度损伤,胆管组织部分纤维增生；而对照组和空白对照组术前与术后对比无明显变化。③术后21天胆道造影发现,实验造模组胆管有狭窄表现；而对照组和空白对照组无胆管狭窄出现。通过上述比较,实验组与对照组差异有统计学意义(P0.05),对照组与空白对照组无统计学意义(P0.05)。结论：通过肝外胆管外滴注95%的乙醇制造大鼠胆管良性狭窄模型是可行的,能够为胆管良性狭窄的研究提供另一种动物模型。
[Abstract]:Objective: to establish a simple, economical and reliable animal model of benign stricture of bile duct in rats, and to study the mechanism and effective treatment of benign stricture of bile duct. Methods: 49 female SD (Sprague Dawley) rats weighing 220-260 g (mean 240 卤10 g) were randomly divided into experimental group (n = 21), experimental control group (n = 21) and blank control group (n = 7). All experimental animals were anesthetized with 3% pentobarbital injection. After abdominal anaesthesia, 21 animals in the experimental group were treated with abdominal anesthesia. The length of the common bile duct was about 1 cm after exposure of the common bile duct, which was about 1 cm from the upper edge of the duodenum, and the length of the common bile duct was about 1 cm from the upper edge of the duodenum. After placing a cotton pad about 0.2cm thick, 95% alcohol was infused into the bile duct for 2-3 minutes and then closed the abdomen. The control group was operated in the same way, and the bile duct was dripped with 0.9% normal saline for 2-3 minutes after abdominal closure, while the blank control group only found the bile duct and closed the abdomen without any other treatment. Postoperative observations included mental state, urine color, and food intake. The experimental model group and the experimental control group were divided into three groups, 7 animals were weighed and the liver function (ALT,GGT,T-BIL,D-BIL) was examined in time, and some of the liver and the bile duct were taken out for pathological examination. Cholangiography was also performed in the 21-day group to find out the biliary obstruction. SPSS software t test and multivariate analysis of variance were used in statistics. Results: after operation, 1hSD rats were able to turn over and drink their own water 3-4 hours later. 1 from pre-operation to 21st day after operation, serum alanine aminotransferase (ALT), was detected in the experimental model group. 纬-glutamyl transferase (纬-glutamyl transferase,GGT), serum total bilirubin (T-BIL) and serum direct bilirubin (D-BIL) were significantly changed (P0.05). However, there were no significant changes in the control group and the blank control group. 2 the rats were killed at each time point to take the liver and bile duct for pathological examination. The experimental model group found that the liver cells were damaged in varying degrees and some of the bile duct fibers proliferated with the passage of time. The contrast between the control group and the blank control group showed no significant changes before and after operation. 3 21 days after operation, bile duct stenosis was found in the experimental model group, but no biliary stricture was found in the control group and the blank control group. Through the above comparison, the difference between the experimental group and the control group was statistically significant (P0.05), while the control group and the blank control group had no statistical significance (P0.05). Conclusion: it is feasible to establish a rat model of benign bile duct stenosis by extrahepatic infusion of 95% ethanol, which can provide another animal model for the study of benign bile duct stenosis.
【学位授予单位】：昆明医学院
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R657.4;R-332

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