溶血磷脂酸对缺氧培养大鼠乳鼠心室肌细胞动作电位及L-型钙电流的影响
发布时间：2018-11-19 20:38
【摘要】：目的： 本实验通过观察外源性溶血磷脂酸(LPA)对缺氧条件下原代培养大鼠乳鼠心室肌细胞动作电位及L-型钙电流的影响,探讨LPA对缺氧培养大鼠乳鼠心室肌细胞的作用。 方法： 应用全细胞膜片钳技术,观察缺氧培养对大鼠乳鼠心室肌细胞动作电位的影响；观察不同剂量LPA对缺氧培养大鼠乳鼠心室肌细胞动作电位及L-型钙电流的影响。 结果： 缺氧培养后的心室肌细胞与正常细胞相比搏动频率减慢、搏动幅度减弱伴有节律不齐和部分停搏,并随培养时间延长程度加重。观察到缺氧培养不同时间后,动作电位的时程随缺氧时间延长而缩短,给予外源性LPA可延长动作电位时程；缺氧培养可使大鼠乳鼠心室肌细胞L-型钙电流的电流幅度明显减小给予外源性LPA使其幅值升高。 结论： 1.缺氧培养大鼠乳鼠心室肌细胞与正常心室肌细胞相比,搏动频率减慢、搏动幅度减弱并伴有节律不齐和部分停搏； 2.缺氧培养条件下大鼠乳鼠心室肌细胞动作电位各参数减小,且具有时间依从性；给予外源性LPA可延长动作电位时程； 3.缺氧培养可使大鼠乳鼠心室肌细胞L-型钙电流减小,且具有时间依从性； 4.LPA能够增强缺氧培养条件下大鼠乳鼠心室肌细胞L-型钙电流的幅值,且具有剂量依赖性。
[Abstract]:Objective: to observe the effects of exogenous lysophosphatidic acid (LPA) on action potential and L-type calcium current in cultured rat ventricular myocytes under hypoxia. To investigate the effect of LPA on ventricular myocytes cultured in neonatal rats. Methods: the effects of hypoxia culture on action potential of ventricular myocytes in neonatal rats and the effects of different doses of LPA on action potential and L-type calcium current of ventricular myocytes in neonatal rats were observed by whole-cell patch clamp technique. Results: compared with the normal cells, the ventricular myocytes cultured under hypoxia had slower pulsatile frequency, lower pulsation amplitude, irregular rhythm and partial arrest, and the degree of pulsation increased with the prolongation of culture time. It was observed that the duration of action potential was shortened with the prolongation of anoxic time after hypoxia culture. Exogenous LPA could prolong the duration of action potential. Hypoxia culture significantly reduced the amplitude of L-type calcium current in neonatal rat ventricular myocytes and increased the amplitude of L- type calcium current by exogenous LPA. Conclusion: 1. Compared with normal ventricular myocytes, hypoxic cultured rat ventricular myocytes had slower pulsatile frequency, lower pulsatile amplitude, irregular rhythm and partial arrest; 2. The parameters of action potential of ventricular myocytes in neonatal rats were decreased in hypoxic culture and had time compliance; exogenous LPA prolonged the duration of action potential; 3. Hypoxia culture could decrease the L-type calcium current in neonatal rat ventricular myocytes, and 4.LPA could enhance the amplitude of L-type calcium current in neonatal rat ventricular myocytes in a dose-dependent manner.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R363
本文编号：2343364
[Abstract]:Objective: to observe the effects of exogenous lysophosphatidic acid (LPA) on action potential and L-type calcium current in cultured rat ventricular myocytes under hypoxia. To investigate the effect of LPA on ventricular myocytes cultured in neonatal rats. Methods: the effects of hypoxia culture on action potential of ventricular myocytes in neonatal rats and the effects of different doses of LPA on action potential and L-type calcium current of ventricular myocytes in neonatal rats were observed by whole-cell patch clamp technique. Results: compared with the normal cells, the ventricular myocytes cultured under hypoxia had slower pulsatile frequency, lower pulsation amplitude, irregular rhythm and partial arrest, and the degree of pulsation increased with the prolongation of culture time. It was observed that the duration of action potential was shortened with the prolongation of anoxic time after hypoxia culture. Exogenous LPA could prolong the duration of action potential. Hypoxia culture significantly reduced the amplitude of L-type calcium current in neonatal rat ventricular myocytes and increased the amplitude of L- type calcium current by exogenous LPA. Conclusion: 1. Compared with normal ventricular myocytes, hypoxic cultured rat ventricular myocytes had slower pulsatile frequency, lower pulsatile amplitude, irregular rhythm and partial arrest; 2. The parameters of action potential of ventricular myocytes in neonatal rats were decreased in hypoxic culture and had time compliance; exogenous LPA prolonged the duration of action potential; 3. Hypoxia culture could decrease the L-type calcium current in neonatal rat ventricular myocytes, and 4.LPA could enhance the amplitude of L-type calcium current in neonatal rat ventricular myocytes in a dose-dependent manner.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R363
【参考文献】
相关博士学位论文 前1条
1 李英骥;溶血磷脂酸对心肌细胞的作用及其病理生理学意义[D];吉林大学;2004年
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