续骨丹外敷疗法对大鼠胫骨缺损模型血清BMP-2的影响
发布时间：2018-11-19 20:26
【摘要】：为观察续骨丹外敷疗法对大鼠胫骨缺损模型血清骨形态发生蛋白-2(BMP-2)表达的影响。给70只SD大鼠右侧胫骨制备1个4 mm缺损,用0.8 mm克氏针内固定。造模1个月后符合标准的大鼠随机分为实验组(药物治疗组)、对照组a(脉冲磁场治疗组)、对照组b(面粉干扰组)和空白组。在干预前、2周、4周后对大鼠右胫骨行X线检查,然后处死动物,收获骨样本,做苏木素-伊红(HE)染色和血清BMP-2检测。干预前,实验组、对照组a、对照组b和空白组,4组大鼠血清BMP-2的含量没有明显差异;干预至第2周时,4组大鼠血清BMP-2的含量与干预前比较均下降,但组间差异均无统计学意义(p0.05);干预4周后,实验组大鼠血清BMP-2的含量高于对照组a、b和空白组,差异均有统计学意义(p0.05),对照组a中BMP-2的含量高于对照组b和空白组,差异均有统计学意义(p0.05),对照组b与空白组比较,差异无统计学意义(p0.05)。4组大鼠血清BMP-2的含量在干预前、2周和4周后的变化趋势是:实验组和对照组a先降后升,对照组b和空白组一直下降。续骨丹外敷疗法和脉冲电磁场疗法在实验干预至第4周时均能上调骨缺损大鼠BMP-2的表达,有利于骨缺损的愈合,但续骨丹外敷疗法对BMP-2的调节作用上明显优于脉冲电磁场。
[Abstract]:To observe the effect of external application of Xuguodan on the expression of serum bone morphogenetic protein-2 (BMP-2) in rat tibial defect model. A 4 mm defect was prepared from the right tibia of 70 SD rats and fixed with 0.8 mm Kirschner needle. One month later, the rats who met the standard were randomly divided into three groups: experimental group (drug treatment group), control group a (pulse magnetic field treatment group), control group b (flour interference group) and blank group. The right tibia of rats was examined by X-ray before, 2 weeks and 4 weeks after intervention, and then the animals were killed, bone samples were harvested, and hematoxylin eosin (HE) staining and serum BMP-2 detection were performed. Before intervention, there was no significant difference in serum BMP-2 content among experimental group, control group b and blank group. At the second week of intervention, the levels of serum BMP-2 in the four groups decreased compared with those before the intervention, but there was no significant difference between the four groups (p0.05). After 4 weeks of intervention, the level of serum BMP-2 in experimental group was higher than that in control group and blank group, the difference was statistically significant (p0.05). The content of BMP-2 in control group a was higher than that in control group and blank group. The difference was statistically significant (p0.05), but there was no significant difference between control group b and blank group (p0.05). The content of serum BMP-2 in the 4 groups was before intervention, and no significant difference was found between the control group and the control group (p0.05). After 2 and 4 weeks, the change trend was: a of the experimental group and the control group decreased first and then increased, and that of the control group b and the blank group decreased all the time. The expression of BMP-2 in rats with bone defect could be upregulated by external application of Xuguodan and pulsed electromagnetic field therapy at the 4th week, which was beneficial to the healing of bone defect. But the effect of external application of Xiegudan on the regulation of BMP-2 was obviously superior to that of pulsed electromagnetic field.
【作者单位】： 南京中医药大学第二临床医学院;南京中医药大学药学院;南京中医药大学第一临床医学院;南京中医药大学中医文献研究所;
【基金】：国家中医药管理局中医药标准化专项基金(ZYYS-2013-(0003)-2)资助
【分类号】：R285.5;R-332
本文编号：2343341
[Abstract]:To observe the effect of external application of Xuguodan on the expression of serum bone morphogenetic protein-2 (BMP-2) in rat tibial defect model. A 4 mm defect was prepared from the right tibia of 70 SD rats and fixed with 0.8 mm Kirschner needle. One month later, the rats who met the standard were randomly divided into three groups: experimental group (drug treatment group), control group a (pulse magnetic field treatment group), control group b (flour interference group) and blank group. The right tibia of rats was examined by X-ray before, 2 weeks and 4 weeks after intervention, and then the animals were killed, bone samples were harvested, and hematoxylin eosin (HE) staining and serum BMP-2 detection were performed. Before intervention, there was no significant difference in serum BMP-2 content among experimental group, control group b and blank group. At the second week of intervention, the levels of serum BMP-2 in the four groups decreased compared with those before the intervention, but there was no significant difference between the four groups (p0.05). After 4 weeks of intervention, the level of serum BMP-2 in experimental group was higher than that in control group and blank group, the difference was statistically significant (p0.05). The content of BMP-2 in control group a was higher than that in control group and blank group. The difference was statistically significant (p0.05), but there was no significant difference between control group b and blank group (p0.05). The content of serum BMP-2 in the 4 groups was before intervention, and no significant difference was found between the control group and the control group (p0.05). After 2 and 4 weeks, the change trend was: a of the experimental group and the control group decreased first and then increased, and that of the control group b and the blank group decreased all the time. The expression of BMP-2 in rats with bone defect could be upregulated by external application of Xuguodan and pulsed electromagnetic field therapy at the 4th week, which was beneficial to the healing of bone defect. But the effect of external application of Xiegudan on the regulation of BMP-2 was obviously superior to that of pulsed electromagnetic field.
【作者单位】： 南京中医药大学第二临床医学院;南京中医药大学药学院;南京中医药大学第一临床医学院;南京中医药大学中医文献研究所;
【基金】：国家中医药管理局中医药标准化专项基金(ZYYS-2013-(0003)-2)资助
【分类号】：R285.5;R-332
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