弗氏枸橼酸杆菌CF74的T6SS影响FliC的表达和分泌

发布时间：2018-11-20 07:14

【摘要】：目的研究弗氏枸橼酸杆菌CF74的T6SS基因组岛功能。方法构建弗氏枸橼酸杆菌CF74的T6SS基因组岛的精确缺失突变株(CF74ΔT6SS)。对弗氏枸橼酸杆菌CF74和其突变株CF74ΔT6SS的全菌蛋白和上清蛋白进行提取,并进行2-DE分析。结果在CF74ΔT6SS全菌蛋白中,有4个蛋白表达上调,42个蛋白表达下调,并且Fli C的表达下调十分明显;在CF74ΔT6SS的培养上清中,有27个蛋白表达上调,36个蛋白表达下调,并且Fli C、Flg K和Fli D的表达下调十分明显。结论弗氏枸橼酸杆菌CF74的T6SS可以影响Fli C蛋白的表达和分泌。
[Abstract]:Objective to study the T6SS genomic island function of Citrobacter flexneri CF74. Methods the CF74 螖 T6SS of the T6SS genomic island of Citrobacter flexneri CF74 was constructed. The whole and supernatant proteins of Citrobacter flexneri CF74 and its mutant CF74 螖 T6SS were extracted and analyzed by 2-DE. Results in the whole bacterial protein of CF74 螖 T6SS, 4 proteins were up-regulated, 42 proteins were down-regulated, and the expression of Fli C was down-regulated. In the culture supernatant of CF74 螖 T6SS, 27 proteins were up-regulated, 36 proteins were down-regulated, and the expression of Fli Cnflg K and Fli D were down-regulated. Conclusion T6SS of Citrobacter flexneri CF74 can affect the expression and secretion of Fli C protein.
【作者单位】：中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室;北京工商大学食品学院;
【基金】：国家自然科学基金(No.81301401) 传染病预防控制国家重点实验室面上项目(No.2011SKLID209)~~
【分类号】：R378.2

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