2-BP对中性粒细胞趋化性的抑制作用

发布时间：2018-11-21 06:50

【摘要】：为研究棕榈酰化对中性粒细胞趋化性的调节作用,利用2-溴棕榈酸(2-bromopalmitate,2-BP)或棕榈酸(palmitate)预处理来源于骨髓的中性粒细胞.采用流式细胞术(flow cytometry,FCM)检测中性粒细胞的存活率和中性粒细胞内F-肌动蛋白(F-actin)的生成;通过免疫荧光分析中性粒细胞内F-actin的极化;利用细胞迁移试验检测中性粒细胞的迁移能力;通过免疫印迹的实验方法检测蛋白激酶B(protein kinase B,AKT)和磷酸化AKT的表达.结果发现,2-BP预处理显著抑制了中性粒细胞内F-actin极化,且抑制了中性粒细胞向趋化肽N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸(N-formylmethionyl-leucyl-phenylalanine,f MLP)迁移的能力,而棕榈酸预处理显著促进了中性粒细胞内F-肌动蛋白极化.两者对F-actin生成和AKT的磷酸化均无影响.研究结果表明,2-BP处理可能通过影响F-actin的极化抑制了中性粒细胞向f MLP的趋化活性.
[Abstract]:In order to study the regulation of palmitoacylation on neutrophil chemotaxis, 2-bromopalmitate 2-BP (2-bromopalmitate 2-BP) or palmitic acid (palmitate) were pretreated from neutrophils from bone marrow. Flow cytometry (flow cytometry,FCM) was used to detect the survival rate of neutrophils and the production of F-actin (F-actin) in neutrophils, and the polarization of F-actin in neutrophils was analyzed by immunofluorescence. The translocation ability of neutrophils was detected by cell migration assay, and the expression of protein kinase (B (protein kinase) and phosphorylated AKT was detected by Western blotting. The results showed that 2-BP pretreatment significantly inhibited the F-actin polarization in neutrophils and the ability of neutrophils to migrate to N-formylmethionyl-leucyl-phenylalanine (MLP). Palmitic acid pretreatment significantly promoted F-actin polarization in neutrophils. Both of them had no effect on F-actin production and AKT phosphorylation. The results show that 2-BP treatment may inhibit the chemotaxis of neutrophils to f MLP by affecting the polarization of F-actin.
【作者单位】：深圳大学医学部中心实验室;聊城大学药学院;上海市第十人民医院中心实验室;同济大学医学院;
【基金】：国家自然科学基金资助项目(31401217) 中国博士后科学基金资助项目(2014M0560672) 深圳市科技基础研究计划资助项目(JCYJ20150403091443312)~~
【分类号】：R392

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