逍遥散对慢性应激大鼠模型血清肿瘤标志物的影响

发布时间：2018-11-21 11:20

【摘要】：目的探讨逍遥散对慢性应激模型大鼠血清肿瘤标志物的影响。方法 Wistar大鼠30只,随机分作模型组、空白组和治疗组,常规饲养,模型组在常规饲养基础上另外给予对应的慢性应激刺激,治疗组在模型组处理基础上给予中药逍遥散煎剂灌胃,21天后采血清,Elisa法测定血清CEA、CA19-9、CA724、AFP。统计学处理,用线性回归分析肿瘤标志物与慢性应激的相关性,方差分析比较三组血清肿瘤标志物差异。结果肿瘤标志物测定值如下,空白组:CEA:9.07±1.43,CA19-9:321.01±35.95,CA724:8.43±1.02,AFP:7.20±0.80;模型组:CEA:22.73±1.6,CA19-9:729.11±57.68,CA724:18.66±1.11,AFP:15.83±0.89;治疗组:CA19-9:521.16±34.73,CA724:12.91±1.18,AFP:7.63±1.20。回归分析提示CA19-9、CA724、AFP与慢性应激有相关性,且以AFP相关性最高。方差分析三组间比较,CA19-9、CA724、AFP三组间差异均有统计学意义(P0.01);LCE-T检验两两组间比较,CA19-9、CA724治疗组与空白组,治疗组与模型组,模型组与空白组之间差异均有统计学意义(P0.01),AFP治疗组与模型组,模型组与空白组之间差异均有统计学意义(P0.01),治疗组与空白组之间比较差异无统计学意义(P0.05)。结论慢性应激损伤能刺激大鼠血清肿瘤标志物升高,有直接诱发肿瘤发生的可能性。慢性应激可能与消化系统肿瘤(特别是肝癌)的发生,关系较为密切。中药方剂逍遥散能够有效降低慢性应激诱发的肿瘤标志物升高,有望预防慢性应激诱发的癌变。
[Abstract]:Objective to investigate the effect of Xiaoyao Powder on serum tumor markers in chronic stress rats. Methods Thirty Wistar rats were randomly divided into three groups: model group, blank group and treatment group. The treatment group was treated with Xiaoyao Powder decoction on the basis of the model group. The serum was collected 21 days later, and the serum CEA,CA19-9,CA724,AFP. was determined by Elisa method. Statistical analysis, linear regression analysis of the correlation between tumor markers and chronic stress, variance analysis of serum tumor markers were compared among the three groups. Results the tumor markers were measured as follows: CEA:9.07 卤1.43CA19-9: 321.01 卤35.95CA7248.43 卤1.02AFP: 7.20 卤0.80; Model group: CEA:22.73 卤1.6 CA19-9: 729.11 卤57.68 CA724: 18.66 卤1.11 AFP: 15.83 卤0.89; treatment group: CA19-9:521.16 卤34.73 CA724: 12.91 卤1.18 AFP: 7.63 卤1.20. Regression analysis showed that there was a correlation between CA19-9,CA724,AFP and chronic stress, and AFP had the highest correlation. There were significant differences in CA19-9,CA724,AFP among the three groups in ANOVA (P0.01). There were significant differences between CA19-9,CA724 treatment group and blank group, treatment group and model group, model group and blank group (P0.01), AFP treatment group and model group, P0.01), AFP treatment group and model group). There were significant differences between the model group and the blank group (P0.01), but there was no significant difference between the treatment group and the blank group (P0.05). Conclusion chronic stress injury can stimulate the increase of serum tumor markers in rats. Chronic stress may be closely related to the occurrence of digestive system tumors, especially liver cancer. Xiaoyao Powder can effectively reduce the increase of tumor markers induced by chronic stress and may prevent carcinogenesis induced by chronic stress.
【作者单位】：宁夏医科大学中医学院宁夏医科大学回医药重点实验室;安徽省池州市人民医院;
【基金】：国家自然科学基金(No.81260515)
【分类号】：R285.5;R-332

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