白假丝酵母菌生物膜形态学及其耐药性研究

发布时间：2018-11-23 14:01

【摘要】：目的:(1)运用荧光显微镜及电镜观察不同阶段白假丝酵母菌生物膜的形态变化。(2)研究白假丝酵母菌生物膜形成过程中其耐药性的变化情况。方法:(1)用载玻片法建立白假丝酵母菌生物膜体外模型,通过荧光探针FITC-ConA标记,荧光显微镜及扫描电镜观察不同阶段生物膜的形态变化。(2)用美国临床实验室标准化委员会(NC-CLS)微量稀释法检测白假丝酵母菌在游离状态、4h BF及48h BF状态下氟康唑的最低抑菌浓度(MIC)。结果:(1)白假丝酵母菌在盖玻片上能够形成典型的BF,通过荧光显微镜及扫描电镜可以看到白假丝酵母菌生物膜随着时间延长其细胞数量及细胞外多糖基质逐渐增多,48h已形成典型的生物膜结构。(2)白假丝酵母菌的耐药性随着生物膜成熟不断增强,其对抗氟康唑的MIC值大小依次为:48h BF4h BF游离状态,且三者之间的差异均有统计学意义。结论:(1)盖玻片法可以很好的构建生物膜体外模型,并可以通过荧光显微镜及扫描电镜检测。(2)白假丝酵母菌其耐药性随着生物膜的成熟逐渐增强。
[Abstract]:Objective: (1) to observe the morphological changes of the biofilm of Candida albicans at different stages by fluorescence microscope and electron microscope, and (2) to study the change of drug resistance during the biofilm formation of Candida albicans. Methods: (1) the biofilm model of Candida albicans was established by slide method and labeled with fluorescent probe FITC-ConA. The morphological changes of biofilms at different stages were observed by fluorescence microscope and scanning electron microscope. (2) Microdilution method was used to detect the free state of Candida albicans by NC-CLS. Minimum inhibitory concentration (MIC).) of fluconazole at 4h BF and 48h BF Results: (1) the typical BF, of Candida albicans could be formed on the cover glass. By fluorescence microscope and scanning electron microscope, the cell number and extracellular polysaccharide matrix of Candida albicans biofilm increased with time. A typical biofilm structure was formed at 48h. (2) the resistance of Candida albicans to fluconazole was increased with the maturation of biofilm, and the MIC value of resistance to fluconazole was as follows: 48h free state of BF4h BF, and the differences among the three were statistically significant. Conclusion: (1) the in vitro biofilm model can be constructed by glass cover method, and it can be detected by fluorescence microscope and scanning electron microscope. (2) the resistance of Candida albicans increases with the maturation of biofilm.
【作者单位】：北京大学第一医院妇产科;暨南大学附属第一医院妇产科;
【分类号】：R378

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