慢性酒精中毒大鼠自由饮模型的建立

发布时间：2018-11-23 18:33

【摘要】：目的采用不同低浓度的酒精饮料让大鼠自由饮用的造模方法,使之建立起与人类慢性酒精中毒类似的简便、理想的大鼠慢性酒精中毒自由饮模型。方法(1)将普通级成年Wistar大鼠(370±30)g,共80只,随机分成对照组1,6%酒精组1,8%酒精组1,12%酒精组1；对照组2,6%酒精组2,8%酒精组2,12%酒精组2,每组各10只。(2)采用逐步递增酒精浓度自由饮法建立慢性酒精中毒动物模型。模型组大鼠每天自由饮用各浓度的酒精饮料,不给于水,造模分两个阶段进行,即3个月和4个月。(3)造模后通过Morris水迷宫实验检测大鼠学习记忆损伤情况,气相色谱法测定血酒精浓度,采用光学显微镜及电子显微镜分别观察酒精中毒后大鼠脑部和肝脏的病理变化。结果(1)同一时间段大鼠自由饮酒精饮料后所摄取到体内的酒精含量以12%酒精组最多,8%酒精组次之,6%酒精组最少；模型组大鼠体重增长缓慢,其中12%酒精组尤为显著。(2)模型组大鼠学习记忆损伤程度在6%酒精组和8%酒精组不明显,12%酒精组最明显。(3)造模3个月和4个月时所测到的模型组大鼠血中酒精浓度基本在150-200mg/100ml之间,其中12%酒精组较高,8%酒精组次之,6%酒精组最少。(4)光镜下3个月时虽然肝脏已有病理变化,但脑部未出现,4个月时脑部出现病理变化；造模4个月后透射电镜下模型组额叶、小脑、海马突触间隙厚度变薄,突触数量减少,突触小泡数量增多,突触间隙变窄,突触厚膜致密物电子密度降低,突触内线粒体双层膜和嵴结构模糊不清或破坏,嵴断裂、变短、减少。结论(1)模型组大鼠自由饮用低浓度酒精饮料3个月,4个月均可以建立慢性酒精中毒大鼠自由饮模型,3个月时只有行为学变化及轻度肝脏病理变化,4个月时脑部出现病理变化,其中12%酒精组最明显。(2)直接测定血液中酒精浓度的方法,可提供准确的大鼠酒精中毒状态。(3)模型组大鼠自由饮用低浓度酒精饮料4个月脑部及肝脏均可出现超微结构改变。
[Abstract]:Objective to establish a simple and ideal rat model of chronic alcohol intoxication similar to human chronic alcoholism by using different low concentration alcoholic drinks to make rats drink freely. Methods (1) 80 adult Wistar rats of general grade (370 卤30) g were randomly divided into control group (n = 16) and control group (n = 1). There were 10 rats in each group in the control group. (2) the animal model of chronic alcoholism was established by stepwise increasing alcohol concentration free drink method. The rats in the model group drank alcohol of different concentrations freely every day and were not given water. The model was made in two stages, that is, 3 months and 4 months. (3) the learning and memory impairment of rats was detected by Morris water maze test. The blood alcohol concentration was measured by gas chromatography. The pathological changes of brain and liver were observed by optical microscope and electron microscope respectively. Results (1) in the same time period, the alcohol content in the body was the highest in 12% alcohol group, the second in 8% alcohol group and the least in 6% alcohol group. The weight of the rats in the model group increased slowly, especially in the 12% alcohol group. (2) the degree of learning and memory impairment in the model group was not significant in the 6% alcohol group and the 8% alcohol group. (3) the concentration of alcohol in the blood of the model group was basically between 150-200mg/100ml after 3 and 4 months of model making, among which 12% alcohol group was higher, 8% alcohol group was the second. 6% alcohol group was the least. (4) there were pathological changes in the liver at 3 months under light microscope, but no changes in the brain, and pathological changes in the brain at 4 months. In the model group, the thickness of synaptic space in frontal lobe, cerebellum and hippocampus became thinner, the number of synapses decreased, the number of synaptic vesicles increased, the synaptic gap narrowed, and the electron density of dense substance of thick synaptic membrane decreased under transmission electron microscope (TEM) 4 months later. The double layer membrane and cristal structure of mitochondria in synapses were blurred or destroyed, the crest was broken, shortened and decreased. Conclusion (1) the rats in the model group were free to drink low-concentration alcoholic drinks for 3 months and 4 months to establish the free drink model of chronic alcoholism. At 3 months, only the behavioral changes and the pathological changes of the liver were observed. Pathological changes occurred in the brain at 4 months, especially in the 12% alcohol group. (2) the method of measuring the concentration of alcohol in blood directly. (3) the rats in the model group had ultrastructural changes in brain and liver after 4 months of free drinking of low-concentration alcoholic beverages.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R-332

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