慢性应激损伤大鼠海马组织全基因组表达谱芯片分析

发布时间：2018-11-25 15:43

【摘要】：目的基于基因表达谱芯片探讨慢性应激引起的基因表达变化,并分析差异基因集合的生物功能及信号通路。方法以Agilent表达谱芯片为载体,检测慢性不可预知性应激大鼠模型及正常对照组的基因表达谱,筛选差异基因,并做基因本体分析和信号通路分析。结果正常对照组与慢性应激模型组相比,已知功能的差异表达基因共106个,其中有56个基因表达上调,涉及视黄醇代谢过程及视觉、氧化还原、ATP结合、辅酶结合等生物过程和分子功能;50个基因表达下调,涉及离子通道合成及活性、细胞-细胞信号、突触传递、脂质合成、γ-氨基丁酸信号通路、γ-氨基丁酸受体活性、细胞膜组成等生物过程和分子功能;KEGG通路分析注释到1个信号通路,即丝裂原活化蛋白激酶(MAPK)通路。结论慢性不可预知性应激刺激导致海马神经元处于应激状态,神经细胞膜的完整性及其周围和内部微环境稳定性发生改变,细胞内各种物质及能量动态平衡可能处于适应性调整状态或已遭到破坏。
[Abstract]:Objective to investigate the changes of gene expression induced by chronic stress based on gene expression microarray, and to analyze the biological function and signal pathway of differential gene set. Methods the gene expression profiles of chronic unpredictable stress rat model and normal control group were detected by using Agilent expression microarray as a vector. The differentially expressed genes were screened, and gene bulk analysis and signal pathway analysis were performed. Results compared with chronic stress model group, there were 106 known differentially expressed genes in normal control group, 56 of which were up-regulated, involving retinol metabolism and visual, redox, ATP binding. Biological processes and molecular functions such as coenzyme binding; The expression of 50 genes is down-regulated, including ion channel synthesis and activity, cell-cell signal, synaptic transmission, lipid synthesis, gamma-aminobutyric acid signaling pathway, gamma-aminobutyric acid receptor activity, cell membrane composition and other biological processes and molecular functions. One signal pathway, mitogen-activated protein kinase (MAPK) pathway, was identified by KEGG pathway analysis. Conclusion chronic unpredictable stress stimulates the hippocampal neurons in stress state and changes the integrity of the membrane and the stability of the surrounding and internal microenvironment. The dynamic balance of various substances and energy in the cell may be in an adaptive state or has been destroyed.
【作者单位】：宁夏医科大学中医学院、宁夏医科大学回医药重点实验室;
【基金】：国家自然科学基金资助项目(编号:81260515)
【分类号】：R363

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