鼻敏感方对变应性鼻炎模型大鼠血清IL-17及IL-10含量的影响

发布时间：2018-11-26 10:50

【摘要】：目的:观察鼻敏感方对变应性鼻炎(AR)模型大鼠的治疗效果及对大鼠血清白介素-17(IL-17)、白介素-10(IL-10)含量的影响,探讨鼻敏感方治疗AR的作用机制。方法:48只SD大鼠随机分为空白对照组,模型组,中药大、中、小剂量组,阳性对照组,共6组。以卵清蛋白(OVA)为致敏原建立大鼠AR模型,造模成功后,模型组灌胃0.9%氯化钠溶液,中药各剂量组灌胃不同浓度的鼻敏感方水提剂,阳性对照组灌胃氯雷他定水溶液,观察各组大鼠行为表现,测定IL-17、IL-10水平,观察鼻黏膜病理形态变化。结果:中药大、中、小剂量组及阳性对照组较模型组鼻痒、喷嚏、清涕等症状明显减轻(P0.05,P0.01),血清IL-17水平显著升高,IL-10水平显著降低(P0.01)。除中药小剂量组外,其余3组鼻黏膜炎性反应程度显著减轻(P0.05,P0.01)。结论:鼻敏感方能有效缓解AR模型大鼠鼻部症状,对其鼻黏膜病理模型具有较好的疗效,其治疗AR可能的机制之一是通过影响IL-17、IL-10水平从而调节Th17/Treg细胞平衡,并且两者呈显著性相关关系,提示IL-17、IL-10在AR发病机制中有重要作用。
[Abstract]:Aim: to observe the therapeutic effect of Bi-sensitive recipe on allergic rhinitis (AR) model rats and its effect on serum levels of interleukin-17 (IL-17) and interleukin-10 (IL-10) in rats, and to explore the mechanism of Bi-sensitive prescription in the treatment of AR. Methods: 48 SD rats were randomly divided into six groups: blank control group, model group, large, medium and small dosage groups and positive control group. The rat model of AR was established by using ovalbumin (OVA) as allergen. After the establishment of the model, the model group was given 0.9% sodium chloride solution, the Chinese medicine groups were given different concentrations of nasal sensitive prescription water extract, and the positive control group was given chloratadine solution. The behavior, IL-17,IL-10 and pathological changes of nasal mucosa were observed. Results: compared with the model group, the symptoms of nasal itching, sneezing and snot in the large, middle and small dosage groups and the positive control group were obviously alleviated (P0.05, P0.01). The level of serum IL-17 increased significantly and the level of IL-10 decreased significantly (P0.01). In addition to the small dose group, the other three groups significantly reduced the degree of nasal inflammatory reaction (P0.05, P0.01). Conclusion: Bi-sensitive prescription can effectively relieve nasal symptoms of AR model rats, and has good curative effect on the pathological model of nasal mucosa. One of the possible mechanisms of its treatment of AR is to regulate the balance of Th17/Treg cells by affecting the level of IL-17,IL-10. There was a significant correlation between the two, suggesting that IL-17,IL-10 plays an important role in the pathogenesis of AR.
【作者单位】：南京中医药大学第一临床医学院;
【基金】：江苏省中医药局暨康缘中医药科技创新基金(No.HZ1001KY)~~
【分类号】：R285.5;R-332

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