虫草多糖对反复脑缺血再灌注模型小鼠学习记忆及脑组织SOD、MDA的影响

发布时间：2018-12-12 20:30

【摘要】：目的:观察虫草多糖反复脑缺血再灌注模型小鼠在虫草多糖干预下对学习记忆及脑组织超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(methane dicarboxylic aldehyde,MDA)的影响。方法:建立反复脑缺血再灌注小鼠模型,采用跳台法和避暗法对小鼠学习记忆能力进行检测,并测定其脑组织SOD活性及MDA含量。结果:与空白组比较,模型组小鼠测试期触电潜伏期显著缩短(P0.01)、训练期及测试期错误反应次数均显著增加(P0.01),说明造模成功。与模型组比较,尼莫地平能够显著延长小鼠触电潜伏期、显著降低小鼠错误反应次数(P0.01);大剂量、中剂量虫草多糖能够显著延长小鼠触电潜伏期(P0.01),显著降低训练期及测试期小鼠跳台错误反应次数(P0.01),小剂量虫草多糖能够明显降低训练期小鼠跳台错误反应次数(P0.05)。与空白组比较,模型组能够显著降低潜伏期(P0.01)、增加错误反应次数(P0.01),说明造模成功。与模型组比较,尼莫地平能够显著延长小鼠潜伏期(P0.01)、减少小鼠错误反应次数(P0.01),大剂量、中剂量、小剂量虫草多糖能够明显增加小鼠潜伏期(P0.01,P0.05),大剂量、中剂量、小剂量虫草多糖能显著降低小鼠错误反应次数(P0.01)。与空白组比较,模型组小鼠脑组织中的SOD活性显著降低(P0.01),MDA含量明显升高(P0.05),说明造模成功。与模型组比较,虫草多糖各剂量组均能明显升高脑缺血小鼠脑组织的SOD活性(P0.05,P0.01),降低MDA含量(P0.05),且与剂量呈正相关性。结论:虫草多糖对脑缺血再灌注损伤模型小鼠的学习记忆能力有一定改善作用,能够提高脑内SOD活性、降低MDA含量,增强自由基的清除能力,具有较好的应用前景。
[Abstract]:Aim: to observe the effects of Cordyceps polysaccharides on learning and memory, superoxide dismutase (superoxide dismutase,SOD) and malondialdehyde (methane dicarboxylic aldehyde,MDA) in cerebral ischemia reperfusion model mice. Methods: the mice model of repeated cerebral ischemia-reperfusion was established. The learning and memory abilities of mice were measured by bench jumping method and dark avoidance method. The activity of SOD and the content of MDA in brain tissue were measured. Results: compared with the blank group, the electric shock latency in the model group was significantly shortened (P0.01), and the number of false reactions during the training period and the test period were significantly increased (P0.01), which indicated that the model was successful. Compared with the model group, nimodipine could significantly prolong the latency of electric shock in mice and decrease the number of wrong reactions in mice (P0.01). Cordyceps polysaccharides in high dose and middle dose could significantly prolong the latency of electric shock (P0.01), and significantly reduce the number of false reaction (P0.01) during the training period and the test period. Low dose Cordyceps polysaccharides could significantly reduce the number of false reaction in mice during training period (P0.05). Compared with the blank group, the model group could significantly reduce the latency (P0.01) and increase the number of errors (P0.01), which indicated that the model was successful. Compared with the model group, nimodipine significantly prolonged the latency of mice (P0.01), decreased the number of errors in mice (P0.01), and significantly increased the latency of mice (P0.01) in large, medium and low dose Cordyceps polysaccharides. P05), large dose, middle dose, low dose Cordyceps polysaccharide can significantly reduce the number of mouse error reaction (P0.01). Compared with the blank group, the activity of SOD in the brain tissue of the model group was significantly decreased (P0.01), MDA content was significantly increased (P0.05), indicating that the model was successful. Compared with the model group, Cordyceps polysaccharides could significantly increase the activity of SOD (P0.05, P0.01) and decrease the content of MDA (P0.05) in the brain tissue of mice with cerebral ischemia, and had a positive correlation with the dose of Cordyceps polysaccharide. Conclusion: Cordyceps polysaccharides can improve the learning and memory ability of mice with cerebral ischemia-reperfusion injury, increase the activity of SOD, decrease the content of MDA and enhance the scavenging ability of free radicals.
【作者单位】：郑州澍青医学高等专科学校;
【基金】：郑州市科技创新团队资助项目(121PCXTD520)
【分类号】：R285.5;R-332

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