丽水市莲都区鼠疫疫源地耶尔森菌的分离及鉴定

发布时间：2018-12-12 22:35

【摘要】：目的了解丽水市莲都区鼠疫疫源地鼠类携带耶尔森菌的类型、毒力基因、生物型以及抗生素敏感等情况。方法利用常规耶尔森菌分离方法从635份鼠类肠道内容物中分离耶尔森菌,对检获的小肠结肠炎耶尔森菌、假结核耶尔森菌进行生物分型试验、抗生素敏感试验和毒力基因检测。结果共检获26株耶尔森菌,其中小肠结肠炎耶尔森菌检出率为2.83%(18/635),均为生物1A型非致病性菌株;假结核耶尔森菌检出率为0.31%(2/635),中间型耶尔森菌检出率为0.31%(2/635),弗氏耶尔森菌检出率为0.47%(3/635),克氏耶尔森检出率为0.16%(1/635)。18株小肠结肠炎耶尔森菌中,2株对庆大霉素耐药,4株对复方新诺明耐药。2株假结核耶尔森菌均对庆大霉素耐药,1株同时对头孢曲松和复方新诺明耐药。结论莲都鼠疫监测点鼠类携带耶尔森菌种类多,以小肠结肠炎耶尔森菌为主,对耶尔森菌的监测要进一步加强,以评估鼠疫暴发的危险度。同时,应警惕耶尔森菌引起的肠道传染病。
[Abstract]:Objective to investigate the types, virulence genes, biotypes and antibiotic sensitivity of Yersinia pestis in plague foci in Liandu district, Lishui City. Methods Yersinia was isolated from 635 rat intestinal contents by routine Yersinia isolation method. The strains of Yersinia enterocolitica and Yersinia pseudotuberculosis were detected by biotyping, antibiotic sensitivity test and virulence gene detection. Results A total of 26 strains of Yersinia enterocolitica were seized. The positive rate of Yersinia enterocolitica was 2.83% (18 / 635). The positive rate of Yersinia pseudotuberculosis was 0.31% (2 / 635), that of Yersinia intermedius was 0.31% (2 / 635), and that of Yersinia flexneri was 0.47% (3 / 635). Among 18 Yersinia enterocolitica strains, 2 strains were resistant to gentamicin, 4 strains were resistant to compound sulfamethoxine, 2 strains of Yersinia pseudotuberculosis were resistant to gentamicin, and 2 strains of Yersinia pseudotuberculosis were resistant to gentamicin. One strain was resistant to both ceftriaxone and compound sulfamethasone. Conclusion Yersinia enterocolitica is the main type of Yersinia enterocolitica in the monitoring sites of Yersinia pestis in Liandu, and the monitoring of Yersinia enterocolitica should be strengthened to assess the risk of Yersinia pestis outbreak. At the same time, we should be on guard against the intestinal infection caused by Yersinia.
【作者单位】：丽水市疾病预防控制中心;
【基金】：浙江省医坛新秀基金资助项目[浙卫发2013(245)号]
【分类号】：R378

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