小鼠初级听皮层SOM中间神经元突触输入的时空特性研究

发布时间：2018-12-15 15:35

【摘要】：目的研究小鼠初级听皮层SOM+神经元接受刺激后突触输入的时空特性。方法通过局部场电位记录方法对初级听皮层进行定位后,采用电压钳全细胞记录方法在离体脑片上分别记录SOM+神经元和锥体神经元在接受刺激后诱发的兴奋后突触后电流和抑制性突触后电流。通过对两类神经元的兴奋性突触后电流和抑制性突触后电流时间特性以及幅度上的比较,从而分析SOM+神经元突触输入的时空特性。结果 SOM+神经元与锥体神经元的突触输入在时间特性上表现出相似的起始潜伏期。SOM+神经元的兴奋性突触后电流和抑制性突触后电流的峰值潜伏期[(14.35±2.74)ms,(18.26±3.24)ms]明显要短于锥体神经元的兴奋性突触后电流和抑制性突触后电流的峰值潜伏期[(18.81±3.76)ms,(21.08±3.93)ms],差异具有统计学意义(EPSC P0.05,IPSC P0.01)。在突触后电流的幅度上两类神经元差异没有统计学意义(P0.05)。结论 SOM+中间神经元接受的突触输入与锥体神经元接受的突触输入在基本电生理特性上类似,但时间特性上的差异提示两者接受的突触输入在皮层内环路来源上可能存在一定差异。
[Abstract]:Objective To study the spatial and temporal characteristics of the postsynaptic input after stimulation of the SOM + neurons in the primary auditory cortex of the mouse. Methods The post-synaptic currents and inhibitory postsynaptic currents induced by SOM + and pyramidal neurons after stimulation were recorded by the method of local field potential recording on the primary auditory cortex, and the post-synaptic currents and the inhibitory postsynaptic currents induced by the stimulation of the SOM + neurons and the pyramidal neurons were recorded on the off-body brain. The time-and-space characteristics of the synaptic inputs of SOM + neurons were analyzed by comparing the excitatory postsynaptic currents and the inhibitory postsynaptic current time characteristics and the amplitude of the two classes of neurons. Results The synaptic inputs of SOM + and pyramidal neurons showed a similar initial incubation period. The peak latency of the excitatory postsynaptic currents and the inhibitory postsynaptic currents of the SOM + neurons[(14.35, 2.74) ms, (18.26, 3.24) ms] is significantly shorter than the peak latency of the excitatory postsynaptic currents and inhibitory postsynaptic currents of the pyramidal neurons[(18.81 to 3.76) ms, (21. 08-3.93) ms], the difference was of statistical significance (EPSC P0.05, IPSC P0.01). There was no significant difference in the amplitude of postsynaptic currents (P0.05). Conclusion The synaptic inputs received by the SOM + intermediate neurons are similar to the synaptic inputs received by the pyramidal neurons, but the difference in time characteristics indicates that the synaptic inputs received by both the SOM + intermediate neurons may be different in the inner loop source of the cortex.
【作者单位】：第三军医大学基础医学部神经生物学教研室重庆市神经生物学重点实验室重庆市神经科学研究所;
【基金】：国家自然科学基金面上项目(31371116,31271177,31471056)~~
【分类号】：R338

【相似文献】