乙型肝炎疫苗联合B7-H1蛋白疫苗对HBV转基因小鼠抗HBsAg免疫应答的影响

发布时间：2018-12-18 11:31

【摘要】：目的:研究B7-H1蛋白疫苗对HBV转基因小鼠免疫应答的影响,探索治疗慢性乙型肝炎的新方法。方法:用不同剂量的乙型肝炎疫苗和B7-H1蛋白疫苗联合免疫HBV转基因小鼠,应用ELISA法检测转基因小鼠在不同时间点血清抗B7-H1抗体滴度,同时在免疫后第14周末处死小鼠取脾细胞,检测不同的免疫方法对小鼠脾细胞产生HBsAg特异性Th1类细胞因子(IFN-γ及IL-2)、对HBsAg特异性分泌IFN-γT细胞数量及对小鼠淋巴细胞增殖的影响。结果:成功完成小鼠的免疫计划,5周起血清中即能测到B7-H1抗体,同一时间点各组之间的抗体滴度值并无明显差异。加B7-H1蛋白免疫各组与相同剂量单用HBsAg蛋白免疫各组相比:IL-2均明显减低(P0.05),分泌IFN-γT的T细胞数量下降,但脾淋巴细胞分泌IFN-γ的水平各组间无明显差异;MTT法测定的淋巴细胞增殖能力各组间也无明显变化。结论:B7-H1蛋白疫苗可诱导HBV转基因小鼠产生明显的抗B7-H1抗体应答,但不能增强抗HBsAg的免疫应答。较小剂量的HBsAg即可引起HBV转基因小鼠Th1类细胞因子(IFN-γ及IL-2)的分泌以及淋巴细胞增殖。
[Abstract]:Aim: to study the effect of B7-H1 protein vaccine on immune response of HBV transgenic mice and to explore a new method for the treatment of chronic hepatitis B. Methods: HBV transgenic mice were immunized with different doses of hepatitis B vaccine and B7-H1 protein vaccine. The titers of anti-B7-H1 antibody in serum of transgenic mice were detected by ELISA method at different time points. At the end of the 14th week after immunization, the mice were killed to take the spleen cells and to detect the production of HBsAg specific Th1 cytokines (IFN- 纬 and IL-2) in the spleen cells of the mice by different immune methods. Effect on the number of IFN- 纬 T cells secreted by HBsAg and on lymphocyte proliferation in mice. Results: B7-H1 antibody could be detected in the serum from 5 weeks after the successful completion of the immunization plan of mice. There was no significant difference in antibody titer between the groups at the same time point. Compared with the same dose groups immunized with HBsAg protein, IL-2 was significantly decreased (P0.05) and the number of T cells secreting IFN- 纬 T was decreased in the groups immunized with B7-H1 protein. However, there was no significant difference in the levels of IFN- 纬 secreted by splenic lymphocytes. The proliferative ability of lymphocytes measured by MTT method also had no significant change among groups. Conclusion: B7-H1 protein vaccine can induce HBV transgenic mice to produce obvious anti B7-H1 antibody response, but can not enhance the immune response against HBsAg. A small dose of HBsAg could induce the secretion of Th1 cytokines (IFN- 纬 and IL-2) and lymphocyte proliferation in HBV transgenic mice.
【作者单位】：第四军医大学唐都医院全军感染病诊疗中心;
【基金】：国家“十一五”传染病重大专项资助(2008ZX10002-008)
【分类号】：R392.12

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