Rb1延缓人脐静脉内皮细胞早熟性衰老与caveolin-1表达的关系
发布时间：2018-12-18 19:05
【摘要】：目的:血管内皮细胞衰老是动脉粥样硬化发生的病理生理机制之一。本研究旨在探讨人参皂苷Rb1延缓人脐静脉内皮细胞(HUVECs)早熟性衰老与窖蛋白1(caveolin-1)表达的关系,为延缓HUVECs衰老提供新的靶点。方法:建立60μmol/L过氧化氢(H_2O_2)诱导的HUVECs早熟性衰老模型,根据细胞形态学的变化、衰老相关β-半乳糖苷酶(SA-β-Gal)染色阳性率和细胞周期评估内皮细胞衰老,采用Western blot和激光共聚焦显微成像的方法检测caveolin-1的变化,观察人参皂苷Rb1对HUVECs衰老的作用及其相关的分子机制。结果:60μmol/L H_2O_2可成功地诱导内皮细胞衰老,早熟性衰老的HUVECs体积变大,SA-β-Gal活性明显增加,细胞发生G_1期阻滞,细胞增殖受抑制,caveolin-1表达增多。与H_2O_2处理组相比,人参皂苷Rb1预处理延缓HUVECs早熟性衰老,SA-β-Gal染色阳性细胞百分比降低,G_0/G_1期细胞比例下降,caveolin-1表达减少。结论:人参皂苷Rb1可通过抑制caveolin-1的表达延缓H_2O_2诱导的HUVECs早熟性衰老。
[Abstract]:Objective: aging of vascular endothelial cells is one of the pathophysiological mechanisms of atherosclerosis. The aim of this study was to investigate the relationship between ginsenoside Rb1 and the expression of cellar protein 1 (caveolin-1) in human umbilical vein endothelial cells (HUVECs), and to provide a new target for delaying HUVECs senescence. Methods: 60 渭 mol/L hydrogen peroxide (H_2O_2) induced HUVECs precocious senescence model was established. Senescence associated 尾 -galactosidase (SA- 尾 -galactosidase) staining and cell cycle were used to evaluate the senescence of endothelial cells. Western blot and confocal laser microscopy were used to detect the changes of caveolin-1. To observe the effect of ginsenoside Rb1 on HUVECs senescence and its molecular mechanism. Results: 60 渭 mol/L H_2O_2 could successfully induce endothelial cell senescence. The HUVECs volume of precocious senescence increased, the activity of SA- 尾-Gal increased significantly, cell G-1 arrest occurred, cell proliferation was inhibited and caveolin-1 expression increased. Compared with H_2O_2 treatment group, ginsenoside Rb1 pretreatment delayed HUVECs precocious senescence, decreased the percentage of SA- 尾-Gal positive cells, decreased the percentage of G_0/G_1 phase cells, and decreased caveolin-1 expression. Conclusion: ginsenoside Rb1 can delay HUVECs senescence induced by H_2O_2 by inhibiting the expression of caveolin-1.
【作者单位】： 中山大学附属第三医院心血管内科;中山大学中西医结合研究所;
【基金】：国家自然科学基金资助项目(No.81370447) 广东省自然科学基金博士启动项目(No.2015A030310048)
【分类号】：R363
本文编号：2386355
[Abstract]:Objective: aging of vascular endothelial cells is one of the pathophysiological mechanisms of atherosclerosis. The aim of this study was to investigate the relationship between ginsenoside Rb1 and the expression of cellar protein 1 (caveolin-1) in human umbilical vein endothelial cells (HUVECs), and to provide a new target for delaying HUVECs senescence. Methods: 60 渭 mol/L hydrogen peroxide (H_2O_2) induced HUVECs precocious senescence model was established. Senescence associated 尾 -galactosidase (SA- 尾 -galactosidase) staining and cell cycle were used to evaluate the senescence of endothelial cells. Western blot and confocal laser microscopy were used to detect the changes of caveolin-1. To observe the effect of ginsenoside Rb1 on HUVECs senescence and its molecular mechanism. Results: 60 渭 mol/L H_2O_2 could successfully induce endothelial cell senescence. The HUVECs volume of precocious senescence increased, the activity of SA- 尾-Gal increased significantly, cell G-1 arrest occurred, cell proliferation was inhibited and caveolin-1 expression increased. Compared with H_2O_2 treatment group, ginsenoside Rb1 pretreatment delayed HUVECs precocious senescence, decreased the percentage of SA- 尾-Gal positive cells, decreased the percentage of G_0/G_1 phase cells, and decreased caveolin-1 expression. Conclusion: ginsenoside Rb1 can delay HUVECs senescence induced by H_2O_2 by inhibiting the expression of caveolin-1.
【作者单位】： 中山大学附属第三医院心血管内科;中山大学中西医结合研究所;
【基金】：国家自然科学基金资助项目(No.81370447) 广东省自然科学基金博士启动项目(No.2015A030310048)
【分类号】：R363
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1 苏冠方，王宜，，王琨, 张晓光，刘早霞;点突变导致Rb1基因内部密码形成终止[J];白求恩医科大学学报;1994年05期
本文编号：2386355
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