鼠伤寒沙门氏菌对肠道屏障的破坏作用

发布时间：2018-12-18 20:41

【摘要】：为了研究鼠伤寒沙门氏菌对肠道屏障的破坏作用,应用小鼠结肠病理组织切片技术、蓝色葡聚糖2000渗透量检测并结合transwell细胞层模型和荧光定量PCR方法探寻鼠伤寒沙门氏菌破坏肠道屏障的可能机制。病理切片结果显示:鼠伤寒沙门氏菌感染可导致肠上皮细胞受损,Babl/c小鼠小肠外蓝色葡聚糖2000渗透量在2、4和6h升高(P0.05),昆明小鼠小肠外其渗透量在2和4h时显著升高(P0.005);细胞跨膜电阻值降低(P0.05);细胞间紧密连接蛋白ZO-1、Claudin-1基因mRNA转录水平下调(P0.005)。结果表明,鼠伤寒沙门氏菌能够通过下调肠上皮细胞紧密连接蛋白的表达来破坏细胞间的紧密连接结构,从而达到破坏肠道屏障的作用。补充阐明了鼠伤寒沙门氏菌感染对肠道屏障的影响,对揭示研究鼠伤寒沙门氏菌的致病机制提供一定依据。
[Abstract]:In order to study the effect of Salmonella typhimurium on the intestinal barrier, the colonic pathological section of mice was used to study the effect of Salmonella typhimurium on intestinal barrier. Blue dextran 2000 osmotic assay combined with transwell cell layer model and fluorescence quantitative PCR method was used to explore the possible mechanism of Salmonella typhimurium destroying intestinal barrier. The results of pathological section showed that the intestinal epithelial cells were damaged by Salmonella typhimurium infection, and the osmotic capacity of blue dextran 2000 in the small intestine of Babl/c mice was increased at 2h and 6h (P0.05). The osmotic capacity of Kunming mice increased significantly at 2 and 4 h (P0.005). The transmembrane resistance was decreased (P0.05) and the mRNA transcription level of intercellular tight junction protein (ZO-1,Claudin-1) gene was down-regulated (P0.005). The results showed that Salmonella typhimurium could destroy the tight junctional structure of the cells by down-regulating the expression of tight junction protein in intestinal epithelial cells, thus destroying the intestinal barrier. The effect of Salmonella typhimurium infection on intestinal barrier was clarified, which provided a basis for the study of pathogenic mechanism of Salmonella typhimurium.
【作者单位】：南昌大学生命科学学院;江西中医药大学基础医学部;南昌大学食品科学与技术国家重点实验室;
【基金】：南昌大学研究生创新专项资金项目(CX2015101)
【分类号】：R378

【相似文献】