淡色库蚊吡丙醚抗性品系和敏感品系羧酸酯酶生化性质差异研究

发布时间：2018-12-25 15:39

【摘要】：目的比较淡色库蚊抗吡丙醚种群和敏感种群羧酸酯酶生化特征的差异,初步探讨其生化抗性机制。方法参照van Aspern(1962)的方法测定羧酸酯酶活性。结果淡色库蚊抗性品系和敏感品系羧酸酯酶水解活性随底物浓度(α-乙酸萘酯或β-乙酸萘酯)的增加而升高;在一定浓度范围内,抗性品系对这两种底物的水解活性均高于敏感品系。β-乙酸萘酯为底物时,抗性品系羧酸酯酶的米氏常数(K_m)和最大反应速度(V_(max))分别为27.20×10~(-5)mol/L和115.00×10~(-5)A/(mg·pro·min),敏感品系K_m和V_(max)分别为104.00×10~(-5)mol/L和207.00×10~(-5)A/(mg·pro·min),两者差异有统计学意义(t_b=2.740,t_c=3.160,P0.05)。α-乙酸萘酯为底物时,抗性品系羧酸酯酶K_m为131.00×10~(-5)mol/L,与敏感品系K_m(75.20×10~(-5)mol/L)比较差异无统计学意义,而V_(max)则差异有统计学意义。胆碱酯酶抑制剂测定结果表明,抗性品系羧酸酯酶对敌敌畏和残杀威的敏感性高于敏感品系。结论羧酸酯酶可能与淡色库蚊对吡丙醚的抗性有关。
[Abstract]:Objective to compare the biochemical characteristics of carboxylesterase of Culex pipiens pallens resistant to pyridine ether and sensitive populations and to explore the biochemical resistance mechanism of Culex pipiens pallens. Methods carboxylesterase activity was determined by van Aspern (1962. Results carboxylesterase hydrolysis activity of resistant and sensitive strains of Culex pipiens pallens increased with the increase of substrate concentration (伪 -naphthalene acetate or 尾 -naphthalene acetate). In a certain concentration range, the hydrolytic activity of the resistant strains to these two substrates was higher than that of the sensitive strains. When 尾 -naphthalene acetate was used as substrate, The Michaelis constant (km) and maximum reaction rate (V _ (max) of carboxylesterase of resistant strains are 27.20 脳 10 ~ (-5) mol/L and 115.00 脳 10 ~ (-5) A / (mg pro min), respectively. For sensitive strains km and V _ (max) were 104.00 脳 10 ~ (-5) mol/L and 207.00 脳 10 ~ (-5) A / (mg pro min), respectively. When 伪 -naphthalene acetate was used as substrate, there was no significant difference between resistant strain Kapm (131.00 脳 10 ~ (-5) mol/L,) and sensitive strain K _ m (75.20 脳 10 ~ (-5) mol/L). The difference of V _ (max) was statistically significant. The results of cholinesterase inhibitor assay showed that the sensitivity of resistant line carboxylesterase to dichlorvos and propoxur was higher than that of sensitive strain. Conclusion carboxylesterase may be related to the resistance of Culex pipiens pallens to pyridine ether.
【作者单位】：上海市疾病预防控制中心病媒生物防治科;
【基金】：上海市自然科学基金(12ZR1426600)~~
【分类号】：R384.1

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