氢醌对骨髓间充质干细胞PARP-1表达的影响

发布时间：2019-01-09 12:31

【摘要】：目的探讨氢醌(HQ)对骨髓间充质干细胞(BMSCs)PARP-1基因表达的影响。方法 2015年1月—2015年3月在东莞市环境医学重点实验室以BMSCs为对照组,以PARP-1沉默BMSCs为处理组,磷酸盐缓冲液(PBS)溶解HQ,分别以0、2.5、5.0、10.0、20.0和40.0μmol/L HQ染毒两组细胞,应用实时荧光定量-聚合酶链反应检测两组细胞PARP-1基因表达的改变。结果与PBS组对比,2.5、5.0、10.0、20.0、40.0μmol/L HQ染毒24 h后,BMSCs细胞PARP-1表达量分别为1.39倍、1.65倍(P0.05)、1.69倍(P0.05)、1.76倍(P0.05)和1.5倍(P0.05);沉默细胞PARP-1表达量分别为1.37倍(P0.05)、1.58倍(P0.05)、1.84倍(P0.05)、2.15倍(P0.05)和1.93倍(P0.05);与BMSCs细胞相比,沉默细胞中PARP-1表达水平下降了78%(P0.05)差异均无统计学意义。结论两组细胞PARP-1表达水平随HQ剂量增加先升后降,PARP-1沉默能增加BMSCs对HQ的敏感性。
[Abstract]:Objective to investigate the effect of hydroquinone (HQ) on (BMSCs) PARP-1 gene expression in bone marrow mesenchymal stem cells. Methods from January 2015 to March 2015, BMSCs was used as control group and PARP-1 silencing BMSCs as treatment group. Phosphate buffer (PBS) dissolved HQ, in Dongguan Environmental Medicine key Laboratory. The changes of PARP-1 gene expression in the two groups were detected by real-time fluorescence quantification-polymerase chain reaction (FQ-PCR). Results compared with PBS group, the expression of PARP-1 in BMSCs cells was 1.39 times, 1.65 times (P0.05), 1.69 times (P0.05), respectively. 1.76 times (P0.05) and 1.5 times (P0.05); The expression of PARP-1 in silencing cells was 1.37 times (P0.05), 1.58 times (P0.05), 1.84 times (P0.05), 2.15 times (P0.05) and 1.93 times (P0.05) respectively. Compared with BMSCs cells, the level of PARP-1 expression in silencing cells decreased by 78% (P0.05). Conclusion the expression of PARP-1 in the two groups increased first with the increase of HQ dose and then decreased. PARP-1 silencing could increase the sensitivity of BMSCs to HQ.
【作者单位】：广东医科大学公共卫生学院劳动卫生与环境卫生教研室;东莞市环境医学重点实验室;
【基金】：广东省自然科学基金资助课题(2015A030310532) 广东省湛江市非资助科技攻关计划课题(2013B01084)
【分类号】：R114

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