B细胞表面TLR2受体在肿瘤来源自噬小体活化B细胞过程中的作用
发布时间：2019-01-14 07:58
【摘要】：目的研究B细胞表面TLR2受体在肿瘤来源自噬小体活化B细胞过程中的作用。方法抗CD43磁珠分选法分离小鼠脾脏B细胞,用不同质量浓度TLR2抗体(0、3、10、30μg/ml)与B细胞共孵育,再用肿瘤来源自噬小体(DRs)刺激B细胞72 h,流式细胞术检测B细胞表面MHCⅡ类分子及共刺激分子CD86的表达情况;第3天收取上清ELISA检测细胞培养上清中IL-6和IL-10的表达;第7天收取上清液,ELISA检测细胞培养上清液中Ig M的表达。结果与未预处理的B细胞组相比,DRs活化TLR2抗体封闭的B细胞组上调B细胞表面MHCⅡ类分子和CD86表达的作用明显降低;DRs刺激TLR2抗体封闭B细胞组分泌IL-6和IL-10的水平显著降低;DRs刺激TLR2抗体封闭的B细胞产生Ig M水平显著低于未预处理的B细胞组。结论 B细胞的TLR2受体在DRs诱导B细胞活化、分泌细胞因子及产生Ig M过程中发挥了重要作用。
[Abstract]:Objective to study the role of TLR2 receptor on B cell surface in the activation of B cells by tumor derived autophagy. Methods B cells of mouse spleen were isolated by anti CD43 magnetic bead sorting method. B cells were incubated with different concentrations of TLR2 antibody (0 10 030 渭 g/ml). B cells were stimulated by tumor derived autophagy (DRs) for 72 h. The expression of MHC class 鈪，
本文编号：2408486
[Abstract]:Objective to study the role of TLR2 receptor on B cell surface in the activation of B cells by tumor derived autophagy. Methods B cells of mouse spleen were isolated by anti CD43 magnetic bead sorting method. B cells were incubated with different concentrations of TLR2 antibody (0 10 030 渭 g/ml). B cells were stimulated by tumor derived autophagy (DRs) for 72 h. The expression of MHC class 鈪，
本文编号：2408486
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