苏铁总黄酮对Lewis肺癌模型小鼠VEGF、bFGF、HIF-1α、NF-κB表达的影响

发布时间：2019-01-14 10:04

【摘要】：目的:探讨苏铁总黄酮对Lewis肺癌模型小鼠血管内皮细胞生长因子(VEGF)、碱性成纤维细胞生长因子(b FGF)、低氧诱导因子-1α(HIF-1α)、核转录因子(NF-κB)表达的影响。方法:采用HE染色法观察肿瘤组织的病理形态变化,免疫组化法和Western blot法检测肿瘤组织中VEGF、b FGF、HIF-1α、NF-κB蛋白的表达,荧光定量PCR法检测肿瘤组织中VEGF、b FGF、HIF-1α、NF-κBmRNA的表达。结果:免疫组化法、Western blot法和荧光定量PCR法的检测结果显示,三者结果基本一致,与模型组比较,苏铁总黄酮各给药组VEGF、b FGF、HIF-1α、NF-κB基因表达量和对VEGF、b FGF、HIF-1α、NF-κB蛋白表达表达率均降低,差异具有显著性(P0.05)。结论:苏铁总黄酮治疗肺癌的作用机制可能为通过抑制侵袭转移相关基因VEGF、b FGF、HIF-1α、NF-κB的表达,进一步抑制侵袭转移相关功能蛋白VEGF、b FGF、HIF-1α、NF-κB的表达,从而发挥抗肺癌侵袭转移作用。
[Abstract]:Objective: to investigate the effects of total flavonoids of cycads on vascular endothelial growth factor (VEGF), basic fibroblast growth factor (b FGF), hypoxia inducible factor-1 伪 (HIF-1 伪) in mice with Lewis lung cancer. Effects of nuclear transcription factor (NF- 魏 B) expression. Methods: the histopathologic changes of tumor tissues were observed by HE staining, the expressions of VEGF,b FGF,HIF-1 伪 and NF- 魏 B in tumor tissues were detected by immunohistochemical method and Western blot method, and VEGF,b FGF, in tumor tissues were detected by fluorescence quantitative PCR method. Expression of HIF-1 伪, NF- 魏 BmRNA. Results: the results of immunohistochemical, Western blot method and fluorescence quantitative PCR method showed that the three results were basically the same. Compared with the model group, the expression of VEGF,b FGF,HIF-1 伪, NF- 魏 B gene and VEGF,b FGF, in the total flavonoids of cycads treated with each group were similar to those of the model group. The expression rate of HIF-1 伪 and NF- 魏 B decreased significantly (P0.05). Conclusion: the mechanism of total flavonoids of cycads in the treatment of lung cancer may be by inhibiting the expression of VEGF,b FGF,HIF-1 伪 and NF- 魏 B, and further inhibiting the invasion and metastasis related functional protein VEGF,b FGF,HIF-1 伪. The expression of NF- 魏 B plays a role in anti-invasion and metastasis of lung cancer.
【作者单位】：中央民族大学中国少数民族传统医学研究院;贵阳中医学院;
【基金】：中央民族大学国家级大学生创新创业训练计划资助项目(GCYS2016110001)
【分类号】：R285.5;R-332

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