皮质酮对海马HT-22细胞株锌含量的影响及机制研究

发布时间：2019-01-18 14:41

【摘要】：目的通过观察皮质酮(corticosterone,CORT)对海马HT-22细胞总锌含量的影响,对其可能机制进行探讨,为研究应激相关疾病提供实验依据。方法小鼠海马神经元HT-22细胞株,经10μmol/L CORT处理6h后,采用原子吸收分光光度计火焰法测定胞内总锌含量,并采用实时荧光定量PCR法检测MT1、MT3、ZnT1、ZnT3和ZIP1m RNA表达。结果与对照组相比,10μmol/L CORT处理HT-22细胞6h后,HT-22细胞内总锌含量显著下降,而先于CORT处理前加入糖皮质激素受体阻断剂RU486组总锌含量与对照组无明显变化。与对照组相比,CORT组HT-22细胞MT1、MT3、ZnT1、ZnT3、ZIP1 mRNA表达水平上升,而先于CORT处理前加入糖皮质激素受体阻断剂RU486组上述基因mRNA表达均无明显变化。结论 CORT可影响海马HT-22细胞锌稳态调节分子的表达,进而影响细胞锌稳态失衡。
[Abstract]:Objective to observe the effect of corticosterone (corticosterone,CORT) on the content of total zinc in hippocampal HT-22 cells and to explore its possible mechanism in order to provide experimental evidence for the study of stress-related diseases. Methods HT-22 cell lines of mouse hippocampal neurons were treated with 10 渭 mol/L CORT for 6 h. The total zinc content in the cells was measured by atomic absorption spectrophotometer flame assay, and the expression of MT1,MT3,ZnT1,ZnT3 and ZIP1m RNA was detected by real-time fluorescence quantitative PCR. Results compared with the control group, the content of total zinc in HT-22 cells decreased significantly after treated with 10 渭 mol/L CORT for 6 h, but the total zinc content in the RU486 group was not significantly different from that in the control group before the treatment with the glucocorticoid receptor blocker RU486. Compared with the control group, the MT1,MT3,ZnT1,ZnT3,ZIP1 mRNA expression level of HT-22 cells in CORT group was increased, but the mRNA expression of these genes in RU486 group did not change significantly before CORT treatment with glucocorticoid receptor blocker RU486. Conclusion CORT can affect the expression of zinc homeostasis molecules in hippocampal HT-22 cells, and then affect the imbalance of zinc homeostasis.
【作者单位】：第二军医大学舰艇卫生学教研室;
【基金】：国家自然科学基金(No.81001243,81573150)
【分类号】：R363

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