舒肌汤对帕金森病肌僵直模型大鼠纹状体多巴胺及其代谢物含量的影响

发布时间：2019-01-22 12:44

【摘要】：目的探讨舒肌汤治疗帕金森病肌僵直的可能作用机理。方法采用颅内注射乳胞素诱导帕金森病肌僵直大鼠模型共24只,随机分为模型组和中药高、中、低剂量组,每组6只,另设假手术组6只(颅内注射生理盐水)。假手术组和模型组均灌胃生理盐水20 g/(kg·d),舒肌汤浓缩至2.0 g/ml,中药高、中、低剂量组分别灌服舒肌汤40、20、10 g/(kg·d),每日1次,持续灌胃30天。检测大鼠纹状体多巴胺(DA)、高香草酸(HVA)和二羟苯乙酸(DOPAC)的含量,电镜观察膈肌神经肌肉接头超微结构改变。结果与假手术组比较,模型组大鼠纹状体DA、HVA、DOPAC含量明显减少,DA/DOPAC和DA/HVA值下降(P0.05或P0.01);与模型组比较,中药中、低剂量组大鼠纹状体DA、HVA、DOPAC含量及DA/DOPAC和DA/HVA值均升高(P0.05或P0.01);与中药中剂量组比较,中药高剂量组DOPAC含量和DA/DOPAC、DA/HVA值下降,中药低剂量组DOPAC含量亦降低(P0.05)。电镜下,模型组存在神经肌肉接头处肌纤维密集,排列紊乱,突触前膜囊泡和线粒体增多,突触后膜皱褶明显,乙酰胆碱受体增多等超微结构改变,中药各剂量组均能不同程度改善上述改变。结论舒肌汤可能通过促进DA分泌,同时减少神经肌肉接头处乙酰胆碱的释放,调节中枢和外周神经兴奋,从而缓解帕金森病肌僵直。
[Abstract]:Objective to explore the possible mechanism of Shuji decoction in the treatment of muscle stiffness of Parkinson's disease. Methods A total of 24 rat models of Parkinson's disease were randomly divided into three groups: model group, high, middle and low dose groups with 6 rats in each group, and sham operation group with 6 rats (intracranial injection of normal saline). Sham operation group and model group were given normal saline 20 g / (kg d), Shuji decoction to 2.0 g / ml. The high, middle and low dose groups were given Shuji decoction 40 ~ 20g / (kg d), once a day for 30 days. The contents of dopamine (DA), homovanillic acid (HVA) and dihydroxyphenylacetate (DOPAC) in rat striatum were measured. The ultrastructural changes of the neuromuscular junction of the diaphragm were observed by electron microscope. Results compared with the sham-operated group, the DA,HVA,DOPAC content in striatum of the model group was significantly decreased, and the DA/DOPAC and DA/HVA values were decreased (P0.05 or P0.01). Compared with the model group, the content of DA,HVA,DOPAC, DA/DOPAC and DA/HVA in the striatum of the low dose group were higher than those in the model group (P0.05 or P0.01). Compared with the middle dose group, the DOPAC content and DA/DOPAC,DA/HVA value in the high dose group decreased, and the DOPAC content in the low dose group also decreased (P0.05). Under electron microscope, there were some ultrastructural changes in the model group, such as the dense and disordered muscle fibers at the neuromuscular junction, the increase of presynaptic vesicles and mitochondria, the obvious postsynaptic membrane folds and the increase of acetylcholine receptors. The above changes were improved in different dosage groups. Conclusion Shuji decoction may promote DA secretion, reduce acetylcholine release at neuromuscular junction, regulate central and peripheral nerve excitation, and relieve muscle stiffness of Parkinson's disease.
【作者单位】：浙江中医药大学基础医学院;
【基金】：浙江省自然科学基金(LY12H27004)
【分类号】：R277.7;R-332

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