苦荞种子中性提取物对链脲佐菌素诱导的2型糖尿病模型大鼠的影响

发布时间：2019-02-17 18:16

【摘要】：目的:研究苦荞种子中性提取物对链脲佐菌素诱导的2型糖尿病模型大鼠的影响。方法:通过高脂高糖饮食4周后35 mg/kg腹腔注射链脲佐菌素(STZ)诱导2型糖尿病大鼠模型。将32只成模大鼠随机均分为模型组、二甲双胍组(156 mg/kg)、苦荞中性提取物(12.8 mg/kg,6.4 mg/kg)组,灌胃给药,每天2次,连续6周。检测大鼠每周体重、饮食、饮水量、尿量,空腹血糖值(FPG);给药6周后,检测大鼠血清胰岛素(INS)与糖化血红蛋白(Hb Alc)水平,计算胰岛素抵抗指数(HOMA-IR);取大鼠胰腺组织,HE染色观察形态学变化。结果:与正常组相比,模型组大鼠饮食、饮水、尿量、FPG、IR、INS、Hb Alc水平升高。与模型组比较,苦荞中性提取物组(12.8 mg/kg、6.4 mg/kg)大鼠饮食、饮水、尿量、FBG,FINS与Hb Alc水平降低。结论:苦荞种子中性提取物可防治由链脲佐菌素诱导的2型糖尿病。
[Abstract]:Aim: to study the effects of neutral extracts from Tartary buckwheat seeds on streptozotocin induced type 2 diabetes rats. Methods: type 2 diabetic rats were induced by intraperitoneal injection of streptozotocin (STZ) at 35 mg/kg after a high fat and high glucose diet for 4 weeks. Thirty-two model rats were randomly divided into two groups: metformin group (156 mg/kg) and Tartary buckwheat neutral extract group (12.8 mg/kg,6.4 mg/kg). The levels of serum insulin (INS) and glycosylated hemoglobin (Hb Alc) and insulin resistance index (HOMA-IR) were measured for 6 weeks after the rats were given weekly body weight, diet, drinking water, urine volume and fasting blood glucose. The pancreatic tissues of rats were taken and the morphologic changes were observed by HE staining. Results: compared with the normal group, the diet, drinking water, urine volume and FPG,IR,INS,Hb Alc level in the model group were increased. Compared with the model group, the level of diet, drinking water, urine volume, FBG,FINS and Hb Alc decreased in the Tartary buckwheat neutral extract group (12. 8 mg/kg,6.4 mg/kg). Conclusion: neutral extract of Tartary buckwheat seed can prevent and cure type 2 diabetes induced by streptozotocin.
【作者单位】：湖北中医药大学中药资源与中药复方教育部重点实验室老年病中药新产品湖北省协同创新中心;劲牌生物医药有限公司;
【基金】：湖北省教育厅青年人才项目基金,项目名称为荞麦治疗糖尿病的物质基础及作用机理研究,项目编号为Q20132007
【分类号】：R285.5;R-332

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