天麻素对不同生理病理状态下CINP大鼠模型的作用
发布时间：2019-02-25 19:36
【摘要】：目的研究天麻素(Gastrodin)对肿瘤、非肿瘤2种不同生理病理状态下化疗诱导的神经病理性疼痛(化疗痛,Chemotherapy-induced Neuropathic Pain,CINP)大鼠模型的作用。方法采用腹腔注射长春新碱(Vincristine,VCR,125μg·kg~(-1))建立正常大鼠和Walker-256乳腺癌荷瘤大鼠CINP动物模型,并腹腔注射给予不同剂量的天麻素(60,120 mg·kg~(-1));采用Von Frey电子测痛仪检测大鼠机械刺激痛阈值,PL-200型热刺痛仪测定大鼠热刺激痛阈值。结果 VCR对肿瘤、非肿瘤大鼠均可诱导产生机械痛敏反应和热痛敏反应,使其痛阈值均明显降低(P0.01);天麻素可剂量依赖性抑制肿瘤、非肿瘤CINP大鼠模型的机械痛敏反应和热痛敏反应(P0.01)。将大鼠注射VCR前后的痛阈值差值以及天麻素治疗前后大鼠痛阈值差值进一步比较,VCR对非肿瘤组大鼠机械痛痛阈值的降低幅度比肿瘤组的明显(P0.01),而VCR对肿瘤组的热痛阈值降低幅度比非肿瘤组明显,但差异无统计学意义(P0.05);天麻素对非肿瘤组大鼠机械痛阈值的提高作用比肿瘤组明显(P0.05),而天麻素对肿瘤组大鼠的热痛阈值提高作用比非肿瘤组明显(P0.05)。结论天麻素对VCR诱导的肿瘤、非肿瘤大鼠CINP均有剂量依赖性抑制作用;但对生理、病理状态下大鼠的不同感觉神经纤维的痛觉抑制作用敏感性存在差异,对VCR诱导痛敏反应越敏感的神经纤维其抑制作用也相对越强。
[Abstract]:Aim to study the effect of gastrodin (Gastrodin) on the neuropathic pain (Chemotherapy-induced Neuropathic Pain,CINP) model induced by chemotherapy in two different physiological and pathological states of tumor and non-tumor. Methods CINP models of normal rats and Walker-256 rats bearing breast cancer were established by intraperitoneal injection of vincristine (Vincristine,VCR, 125 渭 g kg~ (- 1), and different doses of gastrodin (60120 mg kg~ (- 1);) were injected intraperitoneally. The pain threshold of mechanical stimulation in rats was measured by Von Frey electronic pain meter, and the pain threshold of thermal stimulation in rats was measured by PL- 200 hot prickly pain meter. Results VCR could induce mechanical hyperalgesia and thermal hyperalgesia in both tumor and non-tumor rats, resulting in a significant decrease in pain threshold (P0.01). Gastrodin could inhibit the mechanical hyperalgesia and thermal hyperalgesia in tumor-free and non-tumor CINP rats in a dose-dependent manner (P0.01). The difference of pain threshold before and after injection of VCR and the difference of pain threshold before and after gastrodin treatment in rats were further compared. The decrease of mechanical pain threshold in non-tumor group was significantly lower than that in tumor group (P0.01), and the decrease in mechanical pain threshold in non-tumor group was more obvious than that in tumor group (P0.01). However, the decrease of thermal pain threshold in VCR group was more obvious than that in non-tumor group, but there was no significant difference (P0.05). The effect of gastrodin on mechanical pain threshold of non-tumor group was significantly higher than that of tumor group (P0.05), while the effect of gastrodin on thermal pain threshold of non-tumor group was significantly higher than that of non-tumor group (P0.05). Conclusion Gastrodin has a dose-dependent inhibitory effect on VCR-induced tumor and non-tumor rat CINP in a dose-dependent manner. However, there were differences in sensitivity to pain inhibition of different sensory nerve fibers under physiological and pathological conditions. The more sensitive nerve fibers were to VCR-induced hyperalgesia, the stronger their inhibitory effects were.
【作者单位】： 三峡大学医学院国家中药药理三级实验室;宜昌市公安局西陵分局司法监定中心;三峡大学第一临床医学院;
【基金】：湖北省自然科学基金项目(2013CFC066) 湖北省宜昌市科学研究与开发项目(A13301-57)
【分类号】：R285.5;R-332
[Abstract]:Aim to study the effect of gastrodin (Gastrodin) on the neuropathic pain (Chemotherapy-induced Neuropathic Pain,CINP) model induced by chemotherapy in two different physiological and pathological states of tumor and non-tumor. Methods CINP models of normal rats and Walker-256 rats bearing breast cancer were established by intraperitoneal injection of vincristine (Vincristine,VCR, 125 渭 g kg~ (- 1), and different doses of gastrodin (60120 mg kg~ (- 1);) were injected intraperitoneally. The pain threshold of mechanical stimulation in rats was measured by Von Frey electronic pain meter, and the pain threshold of thermal stimulation in rats was measured by PL- 200 hot prickly pain meter. Results VCR could induce mechanical hyperalgesia and thermal hyperalgesia in both tumor and non-tumor rats, resulting in a significant decrease in pain threshold (P0.01). Gastrodin could inhibit the mechanical hyperalgesia and thermal hyperalgesia in tumor-free and non-tumor CINP rats in a dose-dependent manner (P0.01). The difference of pain threshold before and after injection of VCR and the difference of pain threshold before and after gastrodin treatment in rats were further compared. The decrease of mechanical pain threshold in non-tumor group was significantly lower than that in tumor group (P0.01), and the decrease in mechanical pain threshold in non-tumor group was more obvious than that in tumor group (P0.01). However, the decrease of thermal pain threshold in VCR group was more obvious than that in non-tumor group, but there was no significant difference (P0.05). The effect of gastrodin on mechanical pain threshold of non-tumor group was significantly higher than that of tumor group (P0.05), while the effect of gastrodin on thermal pain threshold of non-tumor group was significantly higher than that of non-tumor group (P0.05). Conclusion Gastrodin has a dose-dependent inhibitory effect on VCR-induced tumor and non-tumor rat CINP in a dose-dependent manner. However, there were differences in sensitivity to pain inhibition of different sensory nerve fibers under physiological and pathological conditions. The more sensitive nerve fibers were to VCR-induced hyperalgesia, the stronger their inhibitory effects were.
【作者单位】： 三峡大学医学院国家中药药理三级实验室;宜昌市公安局西陵分局司法监定中心;三峡大学第一临床医学院;
【基金】：湖北省自然科学基金项目(2013CFC066) 湖北省宜昌市科学研究与开发项目(A13301-57)
【分类号】：R285.5;R-332
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